Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
A GTP-DEPENDENT PUSH IS GENERALLY REQUIRED FOR EFFICIENT PROTEIN TRANSLOCATION ACROSS THE MITOCHONDRIAL INNER MEMBRANE INTO THE MATRIX
Autore:
SEPURI NBV; GORDON DM; PAIN D;
Indirizzi:
UNIV PENN,SCH MED,DEPT PHYSIOL,D403 RICHARDS BLDG,3700 HAMILTON WALK PHILADELPHIA PA 19104 UNIV PENN,SCH MED,DEPT PHYSIOL PHILADELPHIA PA 19104
Titolo Testata:
The Journal of biological chemistry
fascicolo: 33, volume: 273, anno: 1998,
pagine: 20941 - 20950
SICI:
0021-9258(1998)273:33<20941:AGPIGR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PURIFIED PRECURSOR PROTEIN; NUCLEOSIDE TRIPHOSPHATES; INTERMEMBRANE SPACE; CYTOPLASMIC CHAPERONES; CHLOROPLASTS REQUIRES; YEAST MITOCHONDRIA; IMPORT MACHINERY; ATP HYDROLYSIS; OUTER-MEMBRANE; HSP70;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
N.B.V. Sepuri et al., "A GTP-DEPENDENT PUSH IS GENERALLY REQUIRED FOR EFFICIENT PROTEIN TRANSLOCATION ACROSS THE MITOCHONDRIAL INNER MEMBRANE INTO THE MATRIX", The Journal of biological chemistry, 273(33), 1998, pp. 20941-20950

Abstract

Mitochondrial biogenesis requires translocation of numerous preproteins across both outer and inner membranes into the matrix of the organelle, This translocation process requires a membrane potential (Delta psi) and ATP, We have recently demonstrated that the efficient import of a urea-denatured preprotein into the matrix requires GTP hydrolysis (Sepuri, N, B, V., Schulke, N,, and Pain, D. (1998) J. Biol, Chem. 273, 1420-1424), We now demonstrate that GTP is generally required for efficient import of various preproteins, both native and urea-denatured,The GTP participation is localized to a particular stage in the protein import process. In the presence of Delta psi but no added nucleoside triphosphates, the transmembrane movement of preproteins proceeds only to a point early in their translocation across the inner membrane. The completion of translocation into the matrix is independent of Delta psi but is dependent on a GTP-mediated ''push,'' This push is Likelymediated by a membrane-bound GTPase on the cis side of the inner membrane. This conclusion is based on two observations: (i) GTP does not readily cross the inner membrane barrier and hence, primarily acts outside the inner membrane to stimulate import, and (ii) the GTP-dependentstage of import does not require soluble constituents of the intermembrane space and can be observed in isolated mitoplasts, Efficient import into the matrix, however, is achieved only through the coordinated action of a cis GTP-dependent push and a trans ATP-dependent ''pull.''

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/04/20 alle ore 14:12:32