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Titolo:
MEASUREMENT OF SULFATE CONCENTRATIONS AND TRACER TRACEE RATIOS IN BIOLOGICAL-FLUIDS BY ELECTROSPRAY TANDEM MASS-SPECTROMETRY/
Autore:
BOISMENU D; ROBITAILLE L; HAMADEH MJ; HONGSPRABHAS P; HOFFER LJ; MAMER OA;
Indirizzi:
MCGILL UNIV,BIOMED MASS SPECTROMETRY UNIT,1130 PINE AVE W MONTREAL PQH3A 1A3 CANADA MCGILL UNIV,JEWISH GEN HOSP,LADY DAVIS INST MED RES MONTREAL PQ H3T 1E2 CANADA MCGILL UNIV,SCH DIETET & HUMAN NUTR ST ANNE BELLEVUE PQ H9X 3V9 CANADA
Titolo Testata:
Analytical biochemistry (Print)
fascicolo: 1, volume: 261, anno: 1998,
pagine: 93 - 99
SICI:
0003-2697(1998)261:1<93:MOSCAT>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
INORGANIC SULFATE; CHROMATOGRAPHY; SULFUR; ADULTS; SODIUM; SERUM; URINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
D. Boismenu et al., "MEASUREMENT OF SULFATE CONCENTRATIONS AND TRACER TRACEE RATIOS IN BIOLOGICAL-FLUIDS BY ELECTROSPRAY TANDEM MASS-SPECTROMETRY/", Analytical biochemistry (Print), 261(1), 1998, pp. 93-99

Abstract

A reproducible and very sensitive method is described for the quantitation of inorganic sulfate in biological fluids by negative electrospray ionization tandem mass spectrometry. After addition to the sample of S-34-labeled sodium sulfate internal standard and deproteinization with methanol, interfering bicarbonate anions are removed by acidification and chloride and phosphate by means of a single filtration step. The tandem mass spectrometer is used in neutral loss mode to detect HSO4- ions free of interference from residual isobaric H2PO4- ions. Organic sulfates do not interfere with the measurement. Serum and urinary inorganic sulfate concentrations measured with this technique agree closely with determinations by ion-exchange chromatography with conductivity detection. Unlike the latter method, this technique does not require dedicated equipment. The method is also suitable for measuring the ratio of S-34-labeled sulfate to unlabeled sulfate in serum and hence represents an attractive alternative for the use of the radioactive S-35 isotope in human studies of body composition and oxidation of sulfur-containing substrates to sulfate. (C) 1998 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 11/07/20 alle ore 21:03:47