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Titolo:
CDNA CLONING OF HUMAN DNASE-GAMMA - CHROMOSOMAL LOCALIZATION OF ITS GENE AND ENZYMATIC-PROPERTIES OF RECOMBINANT PROTEIN
Autore:
SHIOKAWA D; HIRAI M; TANUMA S;
Indirizzi:
SCI UNIV TOKYO,FAC PHARMACEUT SCI,DEPT BIOCHEM,SHINJUKU KU TOKYO 162 JAPAN SCI UNIV TOKYO,FAC PHARMACEUT SCI,DEPT BIOCHEM,SHINJUKU KU TOKYO 162 JAPAN UNIV TOKYO,GRAD SCH SCI,DEPT BIOL SCI,BUNKYO KU TOKYO 113 JAPAN SCI UNIV TOKYO,BIOSCI RES INST CHIBA JAPAN
Titolo Testata:
Apoptosis
fascicolo: 2, volume: 3, anno: 1998,
pagine: 89 - 95
SICI:
1360-8185(1998)3:2<89:CCOHD->2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS ENDONUCLEASE ACTIVATION; CALCIUM-DEPENDENT ENDONUCLEASE; APOPTOTIC RAT THYMOCYTES; CELL-DEATH; CA2+/MG2+-DEPENDENT ENDONUCLEASE; DEOXYRIBONUCLEASE-I; HUMAN XQ28; IDENTIFICATION; PURIFICATION; FRAGMENTATION;
Keywords:
APOPTOSIS; DNA FRAGMENTATION; DNASE GAMMA; ENDONUCLEASE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
D. Shiokawa et al., "CDNA CLONING OF HUMAN DNASE-GAMMA - CHROMOSOMAL LOCALIZATION OF ITS GENE AND ENZYMATIC-PROPERTIES OF RECOMBINANT PROTEIN", Apoptosis, 3(2), 1998, pp. 89-95

Abstract

We report here on the nucleotide sequence of the cDNA encoding human DNase gamma, which is a candidate for an apoptotic endonuclease. The cDNA clone isolated from a human spleen cDNA library is composed of a 918 bp open reading frame encoding a 305 amino acid precursor protein for DNase gamma, Northern blot analysis reveals that the expression of a single transcript of 1.5 kb DNase gamma mRNA is detected in the spleen and liver. The chromosomal localization of DNase gamma gene is mapped to chromosome 3 at region p21.1-p14.2 by fluorescence in situ hybridization (FISH). Characterization of thioredoxin-DNase gamma fusion protein (Trx-hDNase gamma) shows that the recombinant protein has a Ca2+/Mg2+- or Mn2+-dependent endonuclease activity that cleaves chromatin DNA to nucleosomal units. The optimum pH is around 7.2. Zn2+ and aurintricarboxylic acid (ATA) inhibits the activity in dose-dependent manners. These properties are identical to those of purified DNase gamma.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/06/20 alle ore 14:21:03