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Titolo:
INFLUENCE OF PETIOLE AND LAMINA ON ADVENTITIOUS SHOOT INITIATION FROMLEAF EXPLANTS OF PAULOWNIA-FORTUNEI
Autore:
KUMAR PP; RAO CD; GOH CJ;
Indirizzi:
NATL UNIV SINGAPORE,FAC SCI,SCH BIOL SCI,10 KENT RIDGE CRESCENT SINGAPORE 119260 SINGAPORE
Titolo Testata:
Plant cell reports
fascicolo: 11, volume: 17, anno: 1998,
pagine: 886 - 890
SICI:
0721-7714(1998)17:11<886:IOPALO>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
GARCINIA-MANGOSTANA L; CULTURED IN-VITRO; EXCISED LEAVES; REGENERATION;
Keywords:
DUAL-MEDIUM TECHNIQUE; EXPLANT SIZE; PAULOWNIA; PETIOLE CULTURE; SHOOT BUD INITIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
11
Recensione:
Indirizzi per estratti:
Citazione:
P.P. Kumar et al., "INFLUENCE OF PETIOLE AND LAMINA ON ADVENTITIOUS SHOOT INITIATION FROMLEAF EXPLANTS OF PAULOWNIA-FORTUNEI", Plant cell reports, 17(11), 1998, pp. 886-890

Abstract

Adventitious shoot bud differentiation occurred preferentially from the petiolar cut ends of leaf explants of Paulownia fortunei cultured on Murashige and Skoog medium supplemented with 4 mu M alpha-naphthaleneacetic acid and 20 mu M benzyladenine. The details of plantlet regeneration and successful transplantation to soil have been reported earlier. We now show that besides medium supplementation with auxin and cytokinin, the presence of lamina and petiole in the explant influence shoot bud induction. Explants with the basal half of the lamina and the entire petiole were much more responsive than those with whole lamina and petiole. A dual-culture-medium technique which permitted incubation of the two ends of excised petioles under two different phytohormoneregimes was devised. Our data suggest that some of the diffusible factors from the lamina may be phytohormones, and that the establishment of an endogenous phytohormone gradient in the explants may affect shoot bud differentiation in this culture system.

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Documento generato il 01/12/20 alle ore 16:40:43