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Titolo:
NOVEL ANTIGENS FOR NEUROCYSTICERCOSIS - SIMPLE METHOD FOR PREPARATIONAND EVALUATION FOR SERODIAGNOSIS
Autore:
ITO A; PLANCARTE A; MA L; KONG Y; FLISSER A; CHO SY; LIU YH; KAMHAWI S; LIGHTOWLERS MN; SCHANTZ PM;
Indirizzi:
GIFU UNIV,SCH MED,DEPT PARASITOL GIFU 500 JAPAN UNIV NACL AUTONOMA MEXICO,FAC MED,DEPT MICROBIOL & PARASITOL MEXICO CITY DF MEXICO SUNG KYUN KWAN UNIV,COLL MED,DEPT MOL PARASITOL SUWON SOUTH KOREA CATHOLIC UNIV KOREA,SCH MED,DEPT PARASITOL SEOUL SOUTH KOREA CHONGQING UNIV MED SCI,INST INFECT & PARASIT DIS CHONGQING PEOPLES R CHINA YARMOUK UNIV,FAC SCI,DEPT BIOL SCI IRBID JORDAN UNIV MELBOURNE,CTR VET CLIN WERRIBEE VIC AUSTRALIA CTR DIS CONTROL & PREVENT,DIV PARASIT DIS CHAMBLEE GA 00000
Titolo Testata:
The American journal of tropical medicine and hygiene
fascicolo: 2, volume: 59, anno: 1998,
pagine: 291 - 294
SICI:
0002-9637(1998)59:2<291:NAFN-S>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
TAENIA-SOLIUM; SEROLOGICAL DIAGNOSIS; GLYCOPROTEIN ANTIGENS; CYST FLUID; CYSTICERCOSIS; COMPONENTS; ELISA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
16
Recensione:
Indirizzi per estratti:
Citazione:
A. Ito et al., "NOVEL ANTIGENS FOR NEUROCYSTICERCOSIS - SIMPLE METHOD FOR PREPARATIONAND EVALUATION FOR SERODIAGNOSIS", The American journal of tropical medicine and hygiene, 59(2), 1998, pp. 291-294

Abstract

Neurocysticercosis (NCC), which is caused by infection with the larval stage of the pork tapeworm (Taenia solium), is now recognized as a major cause of neurologic diseases in countries where the infection is endemic. Migration of persons from these countries is resulting in diagnosis and local transmission in nonendemic countries at increasing rates. In the present study, immunoblotting and an ELISA were carried out using antigens of T. solium cysticerci fractionated by isoelectric focusing and serum samples from patients with NCC, alveolar (AE) or cystic echinococcosis (CE), and other diseases. Immunoblot analysis revealed antigens fractionated by isoelectric focusing (pH 9.2-9.6) either from cyst fluid of T. solium cysticerci or from intact cysts had unique components (glycoproteins) highly specific and sensitive for detection of NCC exclusively. All confirmed NCC serum samples (53 of 53) recognized at least three major bands of 10-26-kD of fractions with pH 9.2-9.6 from either intact cysts or cyst fluid. These bands were not recognized by sera from patients with other parasitic diseases including AE (0 of 34), CE (0 of 36), or other heterologous parasitoses (0 of 77), patients with hepatoma (0 of 19) or sarcoidosis (0 of 11), or sera from healthy controls (0 of 29). The ELISA using the antigens showed the same sensitivity and specificity for differentiation of NCC (53 of 53) from other diseases (0 of 107) or healthy individuals (0 of 29). Both immunoblotting and the ELISA using the fractionated antigens readily differentiated all NCC from AE or CE in a blind test of 29 serum samples of persons with NCC, CE, and AE. Antigens fractionated from cyst fluid of T. solium cysticerci by a simple, single-step isoelectric focusing (pH 9.2-9.6) are highly specific and sensitive for differential serodiagnosis of NCC in immunoblotting and/or an ELISA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 09:51:03