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Titolo:
INDUCTION OF DIFFERENTIATION OF THE CULTURED RAT MAMMARY EPITHELIAL-CELLS BY TRITERPENE ACIDS
Autore:
PAIK KJ; JEON SS; CHUNG HY; LEE KH; KIM KW; CHUNG JK; KIM ND;
Indirizzi:
PUSAN NATL UNIV,DEPT PHARM PUSAN 609735 SOUTH KOREA PUSAN NATL UNIV,DEPT PHARM PUSAN 609735 SOUTH KOREA PUSAN NATL UNIV,DEPT MOL BIOL PUSAN 609735 SOUTH KOREA PUSAN NATL UNIV,PUSAN CANC RES CTR PUSAN 609735 SOUTH KOREA PUKYONG NATL UNIV,COLL FISHERIES SCI,DEPT AQUAT LIFE MED PUSAN 608737SOUTH KOREA
Titolo Testata:
Archives of pharmacal research
fascicolo: 4, volume: 21, anno: 1998,
pagine: 398 - 405
SICI:
0253-6269(1998)21:4<398:IODOTC>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SKIN TUMOR PROMOTION; RETINOIC ACID; URSOLIC ACID; OLEANOLIC ACID; INHIBITION; 12-O-TETRADECANOYLPHORBOL-13-ACETATE; SUBPOPULATIONS; COMMUNICATION; SAPONINS;
Keywords:
TRITERPENE ACID; URSOLIC ACID; OLEANOLIC ACID; MAMMARY EPITHELIAL CELL; DIFFERENTIATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
K.J. Paik et al., "INDUCTION OF DIFFERENTIATION OF THE CULTURED RAT MAMMARY EPITHELIAL-CELLS BY TRITERPENE ACIDS", Archives of pharmacal research, 21(4), 1998, pp. 398-405

Abstract

We investigated the effects of triterpene acids (TAs), ursolic acid (UA) and oleanolic acid (OA), on the induction of proliferation and differentiation of normal rat mammary epithelial cells (RMEC) or organoids cultured in Matrigel or primary culture system. To elucidate the effects, we tested their differentiation inducing activities with intercellular communication ability, cell cycle patterns, induction of apoptosis, and morphological differentiation in the three dimensional extracellular culture system. To study the changes of RMEC subpopulation in culture, the cultured cells were isolated, immunostained with peanut lectin (PNA) and anti-Thy-1.1 antibody and then analyzed with flow cytometry. Four different subpopulations, such as PNA and Thy-1.1 negativecells (B-), PNA positive cells (PNA+), Thy-1.1 positive cells (Thy-1.1+), PNA and Thy-1.1 positive cells (B+), were obtained and the size of each subpopulation was changed in culture with time in the presence of TAs. Intercellular communication was observed in culture for 7 daysin TAs-treated cells, but not in culture for 4 days with scrape-loading dye transfer technique. G(2)/M phase cells and the number of apoptotic population were increased in TAs-treated groups in cell cycle analyses. S phase fractions were reduced and the change of G(1), phase cells was not observed. The colonies with distinct multicellular structures, such as stellate, ductal, webbed, squamous, lobulo-ductal colonies, were observed in Matrigel culture and the frequencies of each colonywere changed in the presence of TAs. These results suggest that UA and OA have differentiation inducing effects on rat mammary epithelial cells in primary or in Matrigel culture.

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Documento generato il 31/03/20 alle ore 21:29:51