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Titolo:
GENOTOXICITY STUDY OF BOJUNGCHISUP-TANG, AN ORIENTAL HERBAL DECOTION IN-VITRO CHROMOSOME ABERRATION ASSAY IN CHINESE-HAMSTER LUNG-CELLS ANDIN-VIVO SUPRAVITAL-STAINING MICRONUCLEUS ASSAY WITH MOUSE PERIPHERAL RETICULOCYTES
Autore:
RYU JC; KIM KR; KIM HJ; YOUN JY; MYUNG SW; KIM GH; LEE MJ; CHANG IM;
Indirizzi:
KOREA ADV INST SCI & TECHNOL,TOXICOL LAB,POB 131 SEOUL 130650 SOUTH KOREA DONGGUK UNIV,DEPT ORIENTAL MED,SEOCHO KU SEOUL 137064 SOUTH KOREA SEOUL NATL UNIV,INST NAT PROD RES,CHONGRO KU SEOUL 110460 SOUTH KOREA
Titolo Testata:
Archives of pharmacal research
fascicolo: 4, volume: 21, anno: 1998,
pagine: 391 - 397
SICI:
0253-6269(1998)21:4<391:GSOBAO>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
GENE-TOX PROGRAM; BLOOD RETICULOCYTES; MUTAGENICITY TEST; TRANSGENIC MICE; SOS CHROMOTEST; CARCINOGENS; CHEMICALS; ELECTROPHORESIS; MUTATIONS; INDUCTION;
Keywords:
GENOTOXICITY; CLASTOGENICITY; BOJUNGCHISUP-TANG; HERBAL DECOCTION; CHROMOSOME ABERRATION; CHINESE HAMSTER LUNG FIBROBLAST; MICRONUCLEUS; MOUSE PERIPHERAL RETICULOCYTE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
J.C. Ryu et al., "GENOTOXICITY STUDY OF BOJUNGCHISUP-TANG, AN ORIENTAL HERBAL DECOTION IN-VITRO CHROMOSOME ABERRATION ASSAY IN CHINESE-HAMSTER LUNG-CELLS ANDIN-VIVO SUPRAVITAL-STAINING MICRONUCLEUS ASSAY WITH MOUSE PERIPHERAL RETICULOCYTES", Archives of pharmacal research, 21(4), 1998, pp. 391-397

Abstract

The toxicity evaluation of oriental herbal drugs is of great concern at present. Bojungchisuptang (BCST, in Korean), a decocted medicine oforiental herbal mixture, is now well used in clinic at oriental hospitals for the treatment of edema of several diseases in practice. However, the toxicity of the oriental herbal decocted medicines such as genetic toxicity is not well defined until now. In this respect, to clarify the genetic toxicity of BCST, in vitro chromosome aberration assay with Chinese hamster lung (CHL) fibroblasts and in vivo supravital micronucleus assay with mouse peripheral reticulocytes were performed in this study. In the chromosome aberration assay, we used 5,000 mu g/ml BCST as maximum concentration because no remarkable cytotoxicity in CHL cells was observed both in the presence and absence of S-9 metabolicactivation system. No statistical significant differences of chromosome aberrations were observed in CHL cells treated with 5,000, 2,500 and 1,250 mu g/ml BCST for 6 hour both in the presence and absence of S-9 metabolic activation. However, Very weak positive result (6.5 similar to 8.0% aberration) of BCST was obtained in the absence of S-9 metabolic activation system at 5,000 mu g/ml BCST when treated for 24 hour,i.e. 1.5 normal cell cycle time. And also, in vivo clastogenicity of BCST was studied by acridine orange-supravital staining micronucleus assay using mouse peripheral reticulocytes. We used 2,000 mg/kg as the highest oral dose in this micronucleus assay because no acute oral toxicity of BCST was observed in mice. The optimum induction time of micronucleated reticulocytes (MNRETs) was determined as 36 hours after oral administration of 2,000 mg/kg BCST. No significant differences of MNRETs between control and BCST treatment groups were observed in vivo micronucleus assay. From these results, BCST revealed very weak positive result in chromosome aberration assay in vitro with CHL cells and noclastogenicity in micronucleus assay in vivo.

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Documento generato il 29/10/20 alle ore 21:16:22