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Titolo:
RECOMBINATION IN THE 5'-LEADER OF MURINE LEUKEMIA-VIRUS IS ACCURATE AND INFLUENCED BY SEQUENCE IDENTITY WITH A STRONG BIAS TOWARD THE KISSING-LOOP DIMERIZATION REGION
Autore:
MIKKELSEN JG; LUND AH; DUCH M; PEDERSEN FS;
Indirizzi:
AARHUS UNIV,DEPT BIOL MOL & STRUCT,CF MOELLERS ALLE,BLDG 130 DK-8000 AARHUS DENMARK AARHUS UNIV,DEPT BIOL MOL & STRUCT DK-8000 AARHUS DENMARK AARHUS UNIV,DEPT IMMUNOL & MED MICROBIOL DK-8000 AARHUS DENMARK
Titolo Testata:
Journal of virology
fascicolo: 9, volume: 72, anno: 1998,
pagine: 6967 - 6978
SICI:
0022-538X(1998)72:9<6967:RIT5OM>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
INTERTYPIC POLIOVIRUS RECOMBINANTS; HIV-1 REVERSE-TRANSCRIPTASE; GENOMIC RNA; STRAND TRANSFER; AUTOCOMPLEMENTARY SEQUENCE; DEFECTIVE RETROVIRUS; NUCLEOTIDE-SEQUENCE; CROSSOVER REGIONS; INTERNAL REGIONS; INITIATION SITE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
68
Recensione:
Indirizzi per estratti:
Citazione:
J.G. Mikkelsen et al., "RECOMBINATION IN THE 5'-LEADER OF MURINE LEUKEMIA-VIRUS IS ACCURATE AND INFLUENCED BY SEQUENCE IDENTITY WITH A STRONG BIAS TOWARD THE KISSING-LOOP DIMERIZATION REGION", Journal of virology, 72(9), 1998, pp. 6967-6978

Abstract

Retroviral recombination occurs frequently during reverse transcription of the dimeric RNA genome. By a forced recombination approach basedon the transduction of Akv murine leukemia virus vectors harboring a primer binding site knockout mutation and the entire 5' untranslated region, we studied recombination between two closely related naturally occurring retroviral sequences. On the basis of 24 independent template switching events within a 481-nucleotide target sequence containing multiple sequence identity windows, we found that shifting from vectorRNA to an endogenous retroviral RNA template during minus-strand DNA synthesis occurred within defined areas of the genome and did not leadto misincorporations at the crossover site. The nonrandom distribution of recombination sites did not reflect a bias for specific sites dueto selection at the level of marker gene expression. We address whether template snitching is affected by the length of sequence identity, by palindromic sequences, and/or by putative stem-loop structures. Sixteen of 24 sites of recombination colocalized with the kissing-loop dimerization region, and we propose that RNA-RNA interactions between palindromic sequences facilitate template switching. We discuss the putative role of the dimerization domain in the overall structure of the reverse-transcribed RNA dimer and note that related mechanisms of template switching may be found in remote RNA viruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 22:36:58