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Titolo:
EVOLUTIONARY CONSERVATION OF A TESTES-SPECIFIC PROTEASOME SUBUNIT GENE IN DROSOPHILA
Autore:
BELOTE JM; MILLER M; SMYTH KA;
Indirizzi:
SYRACUSE UNIV,DEPT BIOL,130 COLL PL SYRACUSE NY 13244
Titolo Testata:
Gene
fascicolo: 1, volume: 215, anno: 1998,
pagine: 93 - 100
SICI:
0378-1119(1998)215:1<93:ECOATP>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
MELANOGASTER; DIVERGENCE; VIRILIS;
Keywords:
PROS28.1; SPERMATOGENESIS; DROSOPHILA VIRILIS; GENE FAMILY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
24
Recensione:
Indirizzi per estratti:
Citazione:
J.M. Belote et al., "EVOLUTIONARY CONSERVATION OF A TESTES-SPECIFIC PROTEASOME SUBUNIT GENE IN DROSOPHILA", Gene, 215(1), 1998, pp. 93-100

Abstract

Proteasomes are large multisubunit particles that act as the proteolytic machinery for the ubiquitin-dependent proteolytic pathway. The core of this complex, the 20S proteasome, is made up of seven cl-type andseven B-type subunits, arranged in an (alpha 1-alpha 7)(beta 1-beta 7) (beta 1-beta 7)(alpha 1-alpha 7) cofiguration. Previous work had shown that there exist alternative isoforms of the Drosophila melanogaster alpha 4-type subunit, encoded by two distinct genes, alpha 4t1_dm and alpha 4t2_dm, and that these are expressed exclusively in the germline of the testes. We sought to investigate the evolutionary conservation of this phenomenon by screening for orthologs of the alpha 4-type gene family in the distantly related Drosophila species, D. virilis. Weisolated the D. virilis orthologs of the somatically expressed gene, alpha 4_dm, and the testes-specific gene, alpha 4t2_dm. We failed to find an ortholog of the other testes-specific gene, alpha 4t1_dm. The alpha 4_dv gene maps to the X chromosome at 12A-C, its product shares 90% amino acid identity with alpha 4_dm, and it is expressed at high levels in both males and females. The other gene, alpha 4t_dv, encodes aprotein most similar to the testes-specific alpha 4t2_dm proteasome subunit (59% a.a. identity), and it maps to position 27 on chromosome 2. The expression of the alpha 4t_dv gene is testes-specific, like thatof a4t2_dm. The existence of testes-specific alpha 4-type subunits intwo widely diverged subgenera of Drosophila suggests that these subunit isoforms have important functional roles in spermatogenesis. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 12:14:14