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Titolo:
DNA-SEQUENCE MOTIFS ARE ASSOCIATED WITH ABERRANT HOMOLOGOUS RECOMBINATION IN THE MOUSE MACROPHAGE-MIGRATION INHIBITORY FACTOR (MIF) LOCUS
Autore:
KOBAYASHI S; YOSHIDA K; OHSHIMA T; ESUMI N; PARALKAR VM; WISTOW GJ; KULKARNI AB;
Indirizzi:
NIDR,GENE TARGETING RES & CORE FACIL,NIH,BLDG 30,ROOM 132,30 CONVENT DR BETHESDA MD 20892 NIDR,GENE TARGETING RES & CORE FACIL,NIH BETHESDA MD 20892 NEI,SECT MOL STRUCT & FUNCT,NIH BETHESDA MD 20892
Titolo Testata:
Gene
fascicolo: 1, volume: 215, anno: 1998,
pagine: 85 - 92
SICI:
0378-1119(1998)215:1<85:DMAAWA>2.0.ZU;2-5
Fonte:
ISI
Lingua:
ENG
Soggetto:
EMBRYONIC STEM-CELLS; VIRUS 40 RECOMBINANTS; SINGLE-STRANDED-DNA; CVP8-1-P2 ECORI RES; RAT-LIVER; ILLEGITIMATE RECOMBINATION; NUCLEOTIDE-SEQUENCE; GENE; INTEGRATION; BREAKAGE;
Keywords:
GENE TARGETING; TOPOISOMERASE I; AT-RICH SEQUENCE; INTRACISTERNAL-A PARTICLE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
38
Recensione:
Indirizzi per estratti:
Citazione:
S. Kobayashi et al., "DNA-SEQUENCE MOTIFS ARE ASSOCIATED WITH ABERRANT HOMOLOGOUS RECOMBINATION IN THE MOUSE MACROPHAGE-MIGRATION INHIBITORY FACTOR (MIF) LOCUS", Gene, 215(1), 1998, pp. 85-92

Abstract

Homologous recombination is a precise genetic event that can introduce specific alteration in the genome. A planned targeted disruption by homologous recombination of the macrophage migration inhibitory factor(Mif) locus in mouse embryonic stem (ES) cells yielded the targeted clones, some of which had genomic rearrangements inconsistent with the expected homologous recombination event. A detailed characterization of the recombination breakpoints in two of these clones revealed several sequence motifs with possible roles in recombination. These motifs included short regions of sequence identity that may promote DNA. alignment, multiple 5'-AAGG/TTCC-3' tetrameres, topoisomerase I consensus sites, and AT-rich sequences that can promote DNA cleavage and recombination. A retrovirus-like intracisternal-A particle (IAP) family sequence was also identified upstream of the Mif gene, and the LTR of this IAP was involved in one of the recombinations. Identification and characterization of such sequence motifs will be valuable for the gene targeting experiments. (C) 1998 Published by Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 08/04/20 alle ore 08:13:31