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Titolo:
REPRODUCIBILITY OF LSI(R) HER-2 NEU SPECTRUMORANGE(TM) AND CEP-17 SPECTRUMGREEN(TM) DUAL-COLOR DEOXYRIBONUCLEIC-ACID PROBE KIT - FOR ENUMERATION OF GENE AMPLIFICATION IN PARAFFIN-EMBEDDED SPECIMENS - A MULTICENTER CLINICAL VALIDATION-STUDY/
Autore:
MASOOD S; BUI MM; YUNG JF; MARK HFL; WONG EY; BIRKMEIER JM; YANG SJ; HSU P;
Indirizzi:
UNIV FLORIDA,HLTH SCI CTR,DEPT PATHOL,655 W 8TH ST JACKSONVILLE FL 32206 MERCY HOSP CHICAGO IL 60616 MED CTR CHICAGO IL 60616 BROWN UNIV,SCH MED PROVIDENCE RI 02903 RHODE ISL HOSP PROVIDENCE RI 02903 VYSIS DOWNERS GROVE IL 60515
Titolo Testata:
Annals of clinical and laboratory science
fascicolo: 4, volume: 28, anno: 1998,
pagine: 215 - 223
SICI:
0091-7370(1998)28:4<215:ROLHNS>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
BREAST-CANCER; PROGNOSTIC FACTORS; OVEREXPRESSION; EXPRESSION; INSITU;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
7
Recensione:
Indirizzi per estratti:
Citazione:
S. Masood et al., "REPRODUCIBILITY OF LSI(R) HER-2 NEU SPECTRUMORANGE(TM) AND CEP-17 SPECTRUMGREEN(TM) DUAL-COLOR DEOXYRIBONUCLEIC-ACID PROBE KIT - FOR ENUMERATION OF GENE AMPLIFICATION IN PARAFFIN-EMBEDDED SPECIMENS - A MULTICENTER CLINICAL VALIDATION-STUDY/", Annals of clinical and laboratory science, 28(4), 1998, pp. 215-223

Abstract

Overexpression and/or amplification of HER-2/neu gene L-ave been found to be prognostic and predictive in breast and other cancers. Fluorescence in situ hybridization (FISH) assay, with its sensitivity and specificity, can be a superior method of detection when its performance characteristics are demonstrated. A multicenter study was initiated, toevaluate the reproducibility of the LSI HER-2/neu SpectrumOrange and CEP 17 SpectrumGreen Dual Color DNA Probe for enumeration of both the HER-2/neu gene and chromosome 17 (signals) in interphase cells. Section slides were prepared from four cell lines (H, E. R, and N) with known ratios of the HER-2/neu to CEP 17 copy numbers (approximately H = 1.10, E = 1.70, R = 4.50, N = 9.0). The study variable was the ratios ofthe HER-2/neu to chromosome 17 copy numbers. Reproducibility with respect to assay, site, lot, day and reader was evaluated at 3 centers. Out of 120 specimen slides, 100 pet-cent were successfully assayed. There were no significant differences among: (li four repeated assays: ofthe same specimen (p = 0.99), (2) the four probe lots (p = 0.33), or () the four study days (p = 0.54). There were statistically significant, but not important differences among centers and between readers. The ratios of the HER-2/neu to chromosome 17 copy numbers were estimatedwith accuracy and precision; the mean ratios land sd) for specimens, H, E, R, and N were 1.05 (0.06), 1.81 (0.12), 4.48 (0.28), and 8.60 (1.23), respectively. In summary, assays with the LSI HER-2/neu and CEP 17 Dual Color DNA Probe Kit, conducted at three sites by 6 different technicians, over 8 assay days, using kits from four lots; were performed with a high success rate in paraffin-embedded specimens. The signalenumeration was also accurate and precise. This study demonstrated that the results obtained by using the LSI HER-2/neu SpectrumOrange and CEP 17 SpectrumGreen Dual Color DNA Probe Kit are reliable and reproducible.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 18:58:53