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Titolo:
THE RADIOPROTECTIVE EFFECT OF BBI IS ASSOCIATED WITH THE ACTIVATION OF DNA REPAIR-RELEVANT GENES
Autore:
DITTMANN KH; GUEVEN N; MAYER C; RODEMANN HP;
Indirizzi:
EBERHARD KARLS UNIV TUBINGEN,DEPT RADIOTHERAPY,SECT RADIOBIOL & MOL ENVIRONM RES,ROENTGENWEG 11 D-72076 TUBINGEN GERMANY EBERHARD KARLS UNIV TUBINGEN,DEPT RADIOTHERAPY,SECT RADIOBIOL & MOL ENVIRONM RES D-72076 TUBINGEN GERMANY
Titolo Testata:
International journal of radiation biology
fascicolo: 2, volume: 74, anno: 1998,
pagine: 225 - 230
SICI:
0955-3002(1998)74:2<225:TREOBI>2.0.ZU;2-F
Fonte:
ISI
Lingua:
ENG
Soggetto:
P53 TUMOR-SUPPRESSOR; CELL-CYCLE; IONIZING-RADIATION; HUMAN-SKIN; PROTEIN; GADD45; DAMAGE; PCNA; FIBROBLASTS; INHIBITOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
31
Recensione:
Indirizzi per estratti:
Citazione:
K.H. Dittmann et al., "THE RADIOPROTECTIVE EFFECT OF BBI IS ASSOCIATED WITH THE ACTIVATION OF DNA REPAIR-RELEVANT GENES", International journal of radiation biology, 74(2), 1998, pp. 225-230

Abstract

Purpose: To investigate the molecular mechanisms of the radioprotective effect of the Bowman-Birk proteinase inhibitor (BBI) in normal human skin fibroblasts (HSF). Material and methods: The effect of BBI pre-treatment on p53 protein level and on mRNA levels of downstream genes (ERCC3, Gadd45 and p53) was investigated. Results: As indicated by rime-course experiments based on clonogenic assays, a 6 h pre-incubation with BBI before irradiation of HSF with a single dose of 6 Gy resultedin maximum radioprotection. In non-irradiated cells, pre-incubation with BBI resulted in an increased level of p53 protein. Concomitantly, enhanced mRNA levels of the ERCC3 and the Gadd45 genes were observed. As a consequence, BBI-treated cells showed accelerated DNA repair compared with untreated cells when irradiated. Conclusions: The radioprotective effect of the Bowman-Birk proteinase inhibitor was accompanied by elevated mRNA expression of repair-relevant genes prior to irradiation. Activation of the DNA-repair machinery induced by pre-treatment with BBI is one possible mechanism of the radioprotective effect of BBI.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/07/20 alle ore 18:09:34