Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
EVIDENCE OF HEPATOCYTE APOPTOSIS IN RAT-LIVER AFTER THE ADMINISTRATION OF CARBON-TETRACHLORIDE
Autore:
SHI JL; AISAKI K; IKAWA Y; WAKE K;
Indirizzi:
TOKYO MED & DENT UNIV,SCH MED,DEPT ANAT,DIV 1,BUNKYO KU,1-5-45 YUSHIMA TOKYO 113 JAPAN TOKYO MED & DENT UNIV,DIV MED RES,DEPT RETROVIRAL REGULAT,BUNKYO KU TOKYO 113 JAPAN
Titolo Testata:
The American journal of pathology
fascicolo: 2, volume: 153, anno: 1998,
pagine: 515 - 525
SICI:
0002-9440(1998)153:2<515:EOHAIR>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROGRAMMED CELL-DEATH; KUPFFER CELLS; NECROSIS; INJURY; TISSUE; TOXICITY; PROLIFERATION; MACROPHAGES; MECHANISMS; INDUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
J.L. Shi et al., "EVIDENCE OF HEPATOCYTE APOPTOSIS IN RAT-LIVER AFTER THE ADMINISTRATION OF CARBON-TETRACHLORIDE", The American journal of pathology, 153(2), 1998, pp. 515-525

Abstract

In acute liver injury induced by the injection of CCl4, cell death has been attributed to the necrosis of hepatocytes in the centrilobular area. In the present study, we re-examined the hepatic injury evoked by CCl4 in rats and explored the possibility that apoptosis may also contribute to its pathogenesis. Apoptotic hepatocytes were identified and quantified by light and electron microscopy, the in situ immunohistochemical labeling of nuclear DNA fragmentation, flow cytometry, and DNA gel electrophoresis. We found that a substantial number of hepatocytes underwent apoptosis. Apoptotic changes were also observed in ballooned hepatocytes. Apoptotic hepatocytes increased in number at 3 hours and peaked at 6 hours after the CCl4 injection. Apoptotic bodies were sequestrated in. the adjacent hepatocytes and sinusoidal cells. Doublestaining of the cells with immunostaining for phagocytes and terminaldeoxynucleotidyl transferase-mediated dUTP nick end-labeling stainingfor labeling of DNA fragmentation showed that the majority of apoptotic hepatocytes were phagocytosed by Kupffer cells and macrophages. Theresults indicated that apoptosis occurs in the ballooned and injured hepatocytes of the centrilobular area. What occurs after CCl4 administration may be important ia reducing inflammation, shortening the course of acute hepatic injury, and preventing the development of fibrosis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 04:07:55