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Titolo:
MEASUREMENTS OF BACTERIAL PRODUCTION IN COASTAL MARINE ENVIRONMENTS USING LEUCINE - APPLICATION OF A KINETIC APPROACH TO CORRECT FOR ISOTOPE-DILUTION
Autore:
VANLOOIJ A; RIEMANN B;
Indirizzi:
WAGENINGEN UNIV AGR,DEPT NAT CONSERVAT,RITZEMABOSWEG 32A 6700 DD WAGENINGEN NETHERLANDS WATER QUAL RES INST,INT AGCY C-14 DETERMINAT DK-2970 HORSHOLM DENMARK
Titolo Testata:
Marine ecology. Progress series
fascicolo: 1-2, volume: 102, anno: 1993,
pagine: 97 - 104
SICI:
0171-8630(1993)102:1-2<97:MOBPIC>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
NUCLEIC-ACID SYNTHESIS; FREE AMINO-ACIDS; AQUATIC SYSTEMS; MACROMOLECULAR-SYNTHESIS; SEAWATER CULTURES; PROTEIN-SYNTHESIS; GROWTH; BACTERIOPLANKTON; THYMIDINE; RATES;
Keywords:
BACTERIA; ISOTOPE DILUTION; LEUCINE; PRODUCTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
A. Vanlooij e B. Riemann, "MEASUREMENTS OF BACTERIAL PRODUCTION IN COASTAL MARINE ENVIRONMENTS USING LEUCINE - APPLICATION OF A KINETIC APPROACH TO CORRECT FOR ISOTOPE-DILUTION", Marine ecology. Progress series, 102(1-2), 1993, pp. 97-104

Abstract

Bacterial carbon production was measured daily by means of leucine and thymidine incorporation in samples from 2 locations in the eutrophicRoskilde Fjord, Denmark. During periods of 2 wk in March, June, and September 1992, rates of leucine incorporation using 50 nM leucine weresignificantly correlated (r = 0.88, n = 77) to thymidine-based production rates. To correct for external isotope dilution, the maximal rateof leucine incorporation (V(max)) was calculated from additions of subsaturating concentrations of C-14 leucine (1 to 100 nM). During September, the average isotope dilution was 2.7 (SD = 1.4, n = 25) and 2.0 (SD = 0.8, n = 25) times higher than rates obtained from additions of 50 or 100 nM leucine, respectively. The correlation coefficient r between leucine and thymidine increased from 0.79 to 0.88 when leucine incorporation rates were corrected for isotope dilution. In these eutrophic samples, it is necessary to add more than 200 nM to maximize leucine incorporation and to reduce isotope dilution.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:24:10