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Titolo:
AGONIST-INDUCED DESENSITIZATION OF BETA-ADRENOCEPTORS OF BOVINE BRONCHIAL EPITHELIAL-CELLS
Autore:
NOGAMI M; ROMBERGER DJ; RENNARD SI; TOEWS ML;
Indirizzi:
UNIV NEBRASKA,MED CTR,DEPT PHARMACOL,600 S 42ND ST OMAHA NE 68198 UNIV NEBRASKA,MED CTR,DEPT PHARMACOL,600 S 42ND ST OMAHA NE 68198 UNIV NEBRASKA,MED CTR,DEPT INTERNAL MED,PULM SECT OMAHA NE 68105
Titolo Testata:
Clinical science
fascicolo: 5, volume: 85, anno: 1993,
pagine: 651 - 657
SICI:
0143-5221(1993)85:5<651:ADOBOB>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
DDT1 MF-2 CELLS; ADRENERGIC RECEPTORS; PRIMARY CULTURE; SMOOTH-MUSCLE; LUNG; ASSAY;
Keywords:
ADENYLATE CYCLASE; RECEPTOR INTERNALIZATION; RECEPTOR DOWN-REGULATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
27
Recensione:
Indirizzi per estratti:
Citazione:
M. Nogami et al., "AGONIST-INDUCED DESENSITIZATION OF BETA-ADRENOCEPTORS OF BOVINE BRONCHIAL EPITHELIAL-CELLS", Clinical science, 85(5), 1993, pp. 651-657

Abstract

1. Cultured bovine bronchial epithelial cells express a large number of beta-adrenoceptors of the beta2 subtype which are coupled to activation of adenylate cyclase and synthesis of cyclic AMP. The mechanisms involved in agonist-induced desensitization of beta-adrenoceptor function in these cells were investigated. 2. Preincubation of cells with the beta-adrenoceptor agonist isoprenaline induced a rapid desensitization of adenylate cyclase activity stimulated by isoprenaline (40% at 30 min) with little or no decrease in forskolin stimulation or in the number of beta-adrenoceptors detected by radioligand binding, indicating that uncoupling of beta-adrenoceptors from adenylate cyclase had occurred. 3. Uncoupling of beta-adrenoceptors from the stimulatory guanine-nucleotide-binding protein G(s) was indicated by the loss of GTP effects on agonist competition for radioligand binding. Results from subcellular fractionation by sucrose density gradient centrifugation suggest that rapid internalization or sequestration of beta-adrenoceptors occurred along with the desensitization. 4. Preincubation with isoprenaline for longer times led to a marked decrease in total receptor number (85% at 18 h), indicating that receptor down-regulation also occurs. Recovery of receptors after down-regulation was prevented by cycloheximide and by actinomycin D, suggesting that degradation of both receptor protein and receptor mRNA may be involved in down-regulation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/03/20 alle ore 18:49:43