Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
DEVELOPMENT OF A MODEL FOR CLASSIFICATION OF TOXIN-INDUCED LESIONS USING H-1-NMR SPECTROSCOPY OF URINE COMBINED WITH PATTERN-RECOGNITION
Autore:
HOLMES E; NICHOLLS AW; LINDON JC; RAMOS S; SPRAUL M; NEIDIG P; CONNOR SC; CONNELLY J; DAMMENT SJP; HASELDEN J; NICHOLSON JK;
Indirizzi:
UNIV LONDON BIRKBECK COLL,DEPT CHEM,GORDON HOUSE,29 GORDON ST LONDON WC1H 0PP ENGLAND INFOMETRIX INC WOODINVILLE WA 98072 BRUKER ANALYT MESSTECH GMBH D-76287 RHEINSTETTEN GERMANY SMITHKLINE BEECHAM PHARMACEUT,DEPT ANALYT SCI HARLOW CM19 5AD ESSEX ENGLAND GLAXO WELLCOME RES & DEV LTD,DEPT TOXICOL WARE SG12 0DP HERTS ENGLAND
Titolo Testata:
NMR in biomedicine
fascicolo: 4-5, volume: 11, anno: 1998,
pagine: 235 - 244
SICI:
0952-3480(1998)11:4-5<235:DOAMFC>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAGNETIC-RESONANCE SPECTRA; AUTOMATIC DATA REDUCTION; FISCHER-344 RAT; NEPHROTOXICITY; KIDNEY; 1,1,2-TRICHLORO-3,3,3-TRIFLUORO-1-PROPENE; 2-BROMOETHANAMINE; GLUTATHIONE;
Keywords:
BIOMARKER; HEPATOTOXIN; NEPHROTOXIN; NUCLEAR MAGNETIC RESONANCE (NMR) SPECTROSCOPY; PRINCIPAL COMPONENTS ANALYSIS (PCA); SIMCA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
E. Holmes et al., "DEVELOPMENT OF A MODEL FOR CLASSIFICATION OF TOXIN-INDUCED LESIONS USING H-1-NMR SPECTROSCOPY OF URINE COMBINED WITH PATTERN-RECOGNITION", NMR in biomedicine, 11(4-5), 1998, pp. 235-244

Abstract

Pattern recognition approaches were developed and applied to the classification of 600 MHz H-1 NMR spectra of urine from rats dosed with compounds that induced organ-specific damage in either the liver or kidney. Male rats were separated into groups (n = 5) and each treated withone of the following compounds; adriamycin, allyl alcohol, 2-bromoethanamine hydrobromide, hexachlorobutadiene, hydrazine, lead acetate, mercury II chloride, puromycin aminonucleoside, sodium chromate, thioacetamide, 1,1,2-trichloro-3,3,3-trifluoro-1-propene or dose vehicle. Urine samples were collected over a 7 day time-course and analysed using 600 MHz H-1 NMR spectroscopy. Each NMR spectrum was data-reduced to provide 256 intensity-related descriptors of the spectra. Data corresponding to the periods 8-24 h, 24-32 h and 32-56 h post-dose were first analysed using principal components analysis (PCA). In addition, samples obtained 120-144 h following the administration of adriamycin and puromycin were included in the analysis in order to compensate for the fate onset of glomerular toxicity. Having established that toxin-related clustering behaviour could be detected in the first three principal components (PCs), three-quarters of the data were used to construct a soft independent modelling of class analogy (SIMCA) model. The remainder of the data were used as a test set of the model. Only three out of61 samples in the test set were misclassified. Finally as a further test of the model, data from the H-1 NMR spectra of urine from rats that had been treated with uranyl nitrate were used. Successful prediction of the toxicity type of the compound was achieved based on NMR urinalysis data confirming the robust nature of the derived model. (C) 1998John Wiley & Sons, Ltd.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/12/20 alle ore 17:38:35