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Titolo:
USE OF QUASI-EQUIMOLAR, CLOSELY-SPACED, POLY-CO-ION BACKGROUND ELECTROLYTES IN CAPILLARY-ELECTROPHORESIS FOR THE REDUCTION OF ELECTROMIGRATION DISPERSION OVER A WIDE MOBILITY RANGE
Autore:
WILLIAMS RL; CHILDS B; DOSE EV; GUIOCHON G; VIGH G;
Indirizzi:
TEXAS A&M UNIV,DEPT CHEM,POB 300012 COLLEGE STN TX 77842 TEXAS A&M UNIV,DEPT CHEM COLLEGE STN TX 77842 TEXAS A&M UNIV,DEPT COMP SCI COLLEGE STN TX 77843 UNIV TENNESSEE,DEPT CHEM KNOXVILLE TN 37996
Titolo Testata:
Journal of chromatography
fascicolo: 1-2, volume: 814, anno: 1998,
pagine: 199 - 204
Fonte:
ISI
Lingua:
ENG
Soggetto:
ZONE ELECTROPHORESIS; COMPUTER-SIMULATION; SAMPLE ZONES; ISOTACHOPHORETIC SEPARATION; EXPERIMENTAL VALIDATION; CHIRAL SEPARATIONS; EFFICIENCY; DYNAMICS; BEHAVIOR; PROTEINS;
Keywords:
BACKGROUND ELECTROLYTE COMPOSITION; ELECTROMIGRATION DISPERSION; COMPUTER SIMULATION; BENZOIC ACIDS; BENZYL ALCOHOL;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
23
Recensione:
Indirizzi per estratti:
Citazione:
R.L. Williams et al., "USE OF QUASI-EQUIMOLAR, CLOSELY-SPACED, POLY-CO-ION BACKGROUND ELECTROLYTES IN CAPILLARY-ELECTROPHORESIS FOR THE REDUCTION OF ELECTROMIGRATION DISPERSION OVER A WIDE MOBILITY RANGE", Journal of chromatography, 814(1-2), 1998, pp. 199-204

Abstract

The dependence of analyte peak shape and separation efficiency as a function of the mobility spacing of the co-ions was investigated by computer simulation of the electrophoretic process in poly-co-ion background electrolytes. The simulation results indicated that when the mobilities of the strong electrolyte co-ions differed from each other by less than 1.10(-5) cm(2) V-1 s(-1), much of the electromigration dispersion could be eliminated for the analytes that migrated in the mobilitywindow of the co-ion pair. Accordingly, a poly-component, quasi-equimolar mixture of polyethyleneglycol monomethyl ether hydrogensulfates was synthesized in which the effective mobilities of the strong electrolyte anions ranged from about 10.10(-5) to 23.10(-5) cm(2) V-1 s(-1), and the effective mobilities of these anions differed from each other by about 0.5.10(-5) to 1.5.10(-5) cm(2) V-1 s(-1). A comparison of theelectropherograms of the same seven-component sample obtained with a single co-ion background electrolyte and the poly-co-ion background electrolyte (at identical ion strength) verified that the quasi-equimolar, poly-co-ion background electrolyte eliminated much of the peak distortion that was caused by electromigration dispersion and resulted in good separation efficiencies over the entire, broad mobility range covered by the new poly-co-ion background electrolyte. (C) 1998 Elsevier Science B.V. All rights reserved.

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Documento generato il 24/09/20 alle ore 10:55:55