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Titolo:
HUMAN LIVER SLICES EXPRESS THE SAME LIDOCAINE BIOTRANSFORMATION RATE AS ISOLATED HUMAN HEPATOCYTES
Autore:
OLINGA P; MEREMA MT; MEIJER DKF; SLOOFF MJH; GROOTHUIS GMM;
Indirizzi:
UNIV GRONINGEN,CTR PHARM,DEPT PHARMACOL & THERAPEUT,ANTONIUS DEUSINGLAAN 2 9713 AW GRONINGEN NETHERLANDS UNIV GRONINGEN HOSP,DEPT SURG 9713 EZ GRONINGEN NETHERLANDS
Titolo Testata:
ATLA. Alternatives to laboratory animals
fascicolo: 4, volume: 21, anno: 1993,
pagine: 466 - 468
SICI:
0261-1929(1993)21:4<466:HLSETS>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DYNAMIC ORGAN-CULTURE; DRUG-METABOLISM; MAINTENANCE; TRANSPORT;
Keywords:
LIVER SLICES; HEPATOCYTES; LIDOCAINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
13
Recensione:
Indirizzi per estratti:
Citazione:
P. Olinga et al., "HUMAN LIVER SLICES EXPRESS THE SAME LIDOCAINE BIOTRANSFORMATION RATE AS ISOLATED HUMAN HEPATOCYTES", ATLA. Alternatives to laboratory animals, 21(4), 1993, pp. 466-468

Abstract

In order to investigate whether liver slices are a valuable tool for the assessment of drug metabolism in human liver, we compared the phase I metabolism of lidocaine in human liver slices and hepatocytes prepared from three human livers. Lidocaine is mainly metabolised to monoethylglycinexylidide (MEGX) via a cytochrome P450-mediated N-deethylation. The results indicate that the three livers showed considerable inter-individual differences in the rate of formation of MEGX, and that this difference was equally reflected in slices and isolated cells. Theuse of liver slices is still under development, and optimal incubation conditions still need to be assessed. However, these results suggestthat, in slices of 200-300mum thickness, virtually all hepatocytes are involved in the biotransformation of lidocaine, and that the metabolic activity is preserved equally well as in isolated hepatocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:50:10