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Titolo:
INORGANIC-PHOSPHATE CONTENT AND FREE-ENERGY CHANGE OF ATP HYDROLYSIS IN REGIONAL SHORT-TERM HIBERNATING MYOCARDIUM
Autore:
MARTIN C; SCHULZ R; ROSE J; HEUSCH G;
Indirizzi:
UNIV ESSEN GESAMTHSCH KLINIKUM,ZENTRUM INNERE MED,ABT PATHOPHYSIOL D-45122 ESSEN GERMANY UNIV ESSEN GESAMTHSCH KLINIKUM,ZENTRUM INNERE MED,ABT PATHOPHYSIOL D-45122 ESSEN GERMANY
Titolo Testata:
Cardiovascular Research
fascicolo: 2, volume: 39, anno: 1998,
pagine: 318 - 326
SICI:
0008-6363(1998)39:2<318:ICAFCO>2.0.ZU;2-7
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD-FLOW; INOTROPIC STIMULATION; METABOLIC ADAPTATION; CONTRACTILE FAILURE; ISCHEMIC MYOCARDIUM; OXYGEN-CONSUMPTION; CORONARY-OCCLUSION; MODERATE ISCHEMIA; CREATINE-KINASE; RAT HEARTS;
Keywords:
SHORT-TERM HIBERNATION; SWINE; INORGANIC PHOSPHATE; FREE ENERGY OF ATP HYDROLYSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
54
Recensione:
Indirizzi per estratti:
Citazione:
C. Martin et al., "INORGANIC-PHOSPHATE CONTENT AND FREE-ENERGY CHANGE OF ATP HYDROLYSIS IN REGIONAL SHORT-TERM HIBERNATING MYOCARDIUM", Cardiovascular Research, 39(2), 1998, pp. 318-326

Abstract

Objective: Short-term myocardial hibernation is characterized by an adaptation of contractile function to the reduced blood flow, the recovery of creatine phosphate content and lactate balance back towards normal, whereas ATP content remains reduced at a constant level. We examined the hypothesis that, despite: the absence of ATP recovery, the short-term hibernating myocardium regains an energetic balance. Methods: An enzymatic method was modified for the measurement of inorganic phosphate (P-i) in transmural myocardial drill biopsies (about 5 mg). In 12 anaesthetized swine, moderate ischemia was induced by reduction of coronary inflow into the cannulated left anterior descending coronary artery to decrease regional myocardial function (sonomicrometry) by 50%. Results: The development of short-term hibernation was verified by the recovery of creatine phosphate content, the persistence of inotropic reserve in response to dobutamine and the absence of necrosis (triphenyl tetrazolium chloride). At 5-min ischemia, P-i was increased from 3.6 +/- 0.3 (SD) to 8.1 +/- 1.1 mu mol/g(wet wt) (p < 0.05). The free energy of ATP hydrolysis (Delta G(ATP)) was decreased from -57.8 +/- 0.8 to -52.21 +/- 1.4 kJ/mol (p < 0.05). The relationships between function and P-i (r = -0.81) and Delta G(ATP) (r = -0.83), respectively, during control and at 5-min ischemia became invalid at 90-min ischemia,as myocardial blood flow and function remained reduced at a constant level, but P-i decreased back to 4.9 +/- 0.9 mu mol/g (p < 0.05 vs, control and 5-min ischemia): and Delta G(ATP) fully recovered back to -57.2 +/- 1.3 kJ/mol (p < 0.05 vs. 5-min ischemia). Conclusions: In short-term hibernating myocardium, myocardial inorganic phosphate content recovers partially and the free energy change of ATP hydrolysis returns to control values. Contractile function remains reduced by mechanisms other than an energetic deficit. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 16:46:06