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Titolo:
NEIGHBORING BASE DAMAGE-INDUCED BY PERMANGANATE OXIDATION OF 8-OXOGUANINE IN DNA
Autore:
KOIZUME S; INOUE H; KAMIYA H; OHTSUKA E;
Indirizzi:
HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA KU,KITA 12,NISHI 6 SAPPORO HOKKAIDO 060081 JAPAN HOKKAIDO UNIV,FAC PHARMACEUT SCI,KITA KU SAPPORO HOKKAIDO 060081 JAPAN UNIV OCCUPAT & ENVIRONM HLTH,INST IND ECOL SCI,DEPT ENVIRONM ONCOL KITAKYUSHU FUKUOKA 8078555 JAPAN
Titolo Testata:
Nucleic acids research
fascicolo: 15, volume: 26, anno: 1998,
pagine: 3599 - 3607
SICI:
0305-1048(1998)26:15<3599:NBDBPO>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
HA-RAS GENE; ELECTRON-TRANSFER; 8-HYDROXYGUANINE 7,8-DIHYDRO-8-OXOGUANINE; AQUEOUS-SOLUTION; SINGLET OXYGEN; GUANINE; 8-OXO-7,8-DIHYDRO-2'-DEOXYGUANOSINE; 7,8-DIHYDRO-8-OXO-2'-DEOXYGUANOSINE; 8-HYDROXY-2'-DEOXYGUANOSINE; PHOTOOXIDATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
40
Recensione:
Indirizzi per estratti:
Citazione:
S. Koizume et al., "NEIGHBORING BASE DAMAGE-INDUCED BY PERMANGANATE OXIDATION OF 8-OXOGUANINE IN DNA", Nucleic acids research, 26(15), 1998, pp. 3599-3607

Abstract

We found that single-stranded DNA oligomers containing a 7,8-dihydro-8-oxoguanine (8-oxo-G) residue have high reactivity toward KMnO4; the oxidation of 8-oxo-G induces damage to the neighboring nucleotide residues. This paper describes the novel reaction in detail, including experiments that demonstrate the mechanism involved in the induction of DNA damage. The results using DNAs of various base compositions indicated that damaged G, T and C (but not A) sites caused strand scissions after hot piperidine treatment and that the damage around the 8-oxo-G occurred at G sites in both single and double strands with high frequency, The latter substrates were less sensitive to damage. Further, kinetic studies of the KMnO4 reaction of single-stranded oligomers suggested that the reactivity of the DNA bases at the site 5'-adjacent to the8-oxo-G was in the order G > A > T, C, This preference correlates with the electron donating abilities of the bases. In addition, we found that the DNA damage at the G site, which was connected with the 8-oxo-G by a long abasic chain, was inhibited in the above order by the addition of dG, dA or dC. On the other hand, the damage reactions proceeded even after the addition of scavengers for active oxygen species. This study suggests the involvement of a redox process in the unique DNA damage initiated by the oxidation of the 8-oxo-G.

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Documento generato il 11/07/20 alle ore 10:39:19