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Titolo:
SPECIFICITY AND SYNERGISM OF POLYPEPTIDE GROWTH-FACTORS IN STIMULATING THE SYNTHESIS OF PROTEOGLYCANS AND A NOVEL HIGH-MOLECULAR-WEIGHT ANIONIC GLYCOPROTEIN BY ARTICULAR CHONDROCYTE CULTURES
Autore:
CHOPRA R; ANASTASSIADES T;
Indirizzi:
QUEENS UNIV,DEPT MED,ETHERINGTON HALL KINGSTON ON K7L 3N6 CANADA QUEENS UNIV,DEPT MED KINGSTON ON K7L 3N6 CANADA QUEENS UNIV,DEPT BIOCHEM KINGSTON ON K7L 3N6 CANADA QUEENS UNIV,RHEUMAT DIS UNIT KINGSTON ON K7L 3N6 CANADA
Titolo Testata:
Journal of rheumatology
fascicolo: 8, volume: 25, anno: 1998,
pagine: 1578 - 1584
SICI:
0315-162X(1998)25:8<1578:SASOPG>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
FACTOR-BETA; TGF-BETA; CARTILAGE; BONE; METABOLISM; RECEPTORS; PLATE; ADULT; CALF;
Keywords:
POLYPEPTIDE GROWTH FACTORS; PROTEOGLYCANS; ARTICULAR CARTILAGE; GLYCOPROTEINS; CARTILAGE REPAIR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
R. Chopra e T. Anastassiades, "SPECIFICITY AND SYNERGISM OF POLYPEPTIDE GROWTH-FACTORS IN STIMULATING THE SYNTHESIS OF PROTEOGLYCANS AND A NOVEL HIGH-MOLECULAR-WEIGHT ANIONIC GLYCOPROTEIN BY ARTICULAR CHONDROCYTE CULTURES", Journal of rheumatology, 25(8), 1998, pp. 1578-1584

Abstract

Objective, To determine the specificity of and possible synergism among polypeptide growth factors (PGF) on the net synthesis of proteoglycan and a novel high molecular weight anionic glycoprotein (HMW AG), ofroughly 540 kDa, by articular chondrocyte cultures. Methods. Confluent articular chondrocyte cultures were labeled with either [S-35]SO4 or[H-3]glucosamine and [S-35]SO4 and stimulated by adding the individual PGF or combinations of the PGF. Alcian blue dye precipitation was used for direct rapid quantification of newly synthesized proteoglycans from the media of the S-35 labeled cultures. To assess the effects of the PGF on the synthesis of the HMW AGI a toluidine blue dye batch precipitation method was used for isolation of anionic glycoconjugates from the media of the H-3 and S-35 labeled articular chondrocyte cultures, followed by chondroitinase ABC digestion and gradient sodium dodecyl sulfate polyacrylamide gel electrophoresis. Results, Optimal concentrations of transforming growth factor-beta 1 (TGF-beta), insulin-like growth factor-1 (IGF), and platelet derived growth factor (PDGF) stimulated net proteoglycan synthesis per cell by 178, 106, and 101%, respectively. In combinations, TGF-beta + PDGF, TGF-beta + IGF and PDGF + IGF gave stimulations of proteoglycan synthesis of 418, 384, and 217%, respectively, HMW AG net synthesis was induced by all 3 PGF but the degree of induction was much greater with TGF-beta. Conclusion, Net proteoglycan synthesis by articular chondrocyte cultures is synergistically stimulated by the addition of optimal concentrations of combinationsof TGF-beta + PDGF and TGF-beta + IGF, but not PDGF + IGE Net HMW AG synthesis is preferentially stimulated by TGF-beta.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 06:50:06