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Titolo:
ATTACHMENT OF PC12 CELLS TO ADHESION SUBSTRATUM INDUCES THE ACCUMULATION OF GLUCOSE TRANSPORTERS (GLUTS) AND STIMULATES GLUCOSE-METABOLISM
Autore:
DWYER DS; PINKOFSKY HB; LIU Y; BRADLEY RJ;
Indirizzi:
LOUISIANA STATE UNIV,MED CTR,DEPT PSYCHIAT,1501 KINGS HIGHWAY SHREVEPORT LA 71130 LOUISIANA STATE UNIV,MED CTR,DEPT PHARMACOL & THERAPEUT SHREVEPORT LA71130
Titolo Testata:
Neurochemical research
fascicolo: 8, volume: 23, anno: 1998,
pagine: 1107 - 1116
SICI:
0364-3190(1998)23:8<1107:AOPCTA>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
RAT-BRAIN; GROWTH-FACTOR; DEVELOPMENTAL EXPRESSION; MURINE FIBROBLASTS; EMBRYO FIBROBLASTS; 3T3-L1 ADIPOCYTES; GENE-EXPRESSION; MOUSE EMBRYOS; HEXOSE UPTAKE; IN-VIVO;
Keywords:
GLUCOSE TRANSPORTERS; GLUT3; PC12 CELLS; NEURONAL DIFFERENTIATION; POLY-L-LYSINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
D.S. Dwyer et al., "ATTACHMENT OF PC12 CELLS TO ADHESION SUBSTRATUM INDUCES THE ACCUMULATION OF GLUCOSE TRANSPORTERS (GLUTS) AND STIMULATES GLUCOSE-METABOLISM", Neurochemical research, 23(8), 1998, pp. 1107-1116

Abstract

The levels of glucose transporters (GLUTs), specifically GLUT3 and GLUT1, increased dramatically in PC12 cells that were cultured on suitable adhesion substrate (poly-l-lysine [PLL]) and induced to differentiate with nerve growth factor (NGF). Closer examination of this responserevealed that: (1) cellular attachment to PLL was sufficient to stimulate the increase in GLUT immunoreactivity, and (2) NGF alone was not effective unless the cells were cultured on PLL-treated surfaces. The response to PLL was detected as early as 4 hr after plating the cells and peaked within 24-48 hr. Other adhesion substrata, such as collagenand poly-l-ornithine, evoked a similar response, although the latter polymer was far less effective. The increase in GLUTs appeared to result from an accumulation of existing transporters because this responsewas not blocked by inhibiting protein synthesis. Cellular adhesion toPLL was also accompanied by a rapid activation of glucose metabolism. Thus, specific recognition of the adhesion substratum not only provides a context for cell attachment, but also elicits important functional changes in GLUT activity.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 18:02:04