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Titolo:
AFFINITY CAPILLARY-ELECTROPHORESIS IN BIOMOLECULAR RECOGNITION
Autore:
CHU YH; CHENG CC;
Indirizzi:
OHIO STATE UNIV,DEPT CHEM,100 W 18TH AVE COLUMBUS OH 43210
Titolo Testata:
Cellular and molecular life sciences
fascicolo: 7, volume: 54, anno: 1998,
pagine: 663 - 683
SICI:
1420-682X(1998)54:7<663:ACIBR>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
LASER-INDUCED FLUORESCENCE; IONIZATION MASS-SPECTROMETRY; SPECTROSCOPIC DETECTION SYSTEM; ION-SELECTIVE MICROELECTRODES; PULSED AMPEROMETRIC DETECTION; BOVINE CARBONIC-ANHYDRASE; MEASURE BINDING CONSTANTS; ZONE ELECTROPHORESIS; ELECTROSPRAY-IONIZATION; SINGLE-MOLECULE;
Keywords:
AFFINITY CAPILLARY ELECTROPHORESIS; RECEPTOR-LIGAND BINDING INTERACTION; MOLECULAR RECOGNITION; COMBINATORIAL LIBRARY SCREENING; PROTEIN;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
140
Recensione:
Indirizzi per estratti:
Citazione:
Y.H. Chu e C.C. Cheng, "AFFINITY CAPILLARY-ELECTROPHORESIS IN BIOMOLECULAR RECOGNITION", Cellular and molecular life sciences, 54(7), 1998, pp. 663-683

Abstract

Affinity capillary electrophoresis is a new method for studies of biomolecular recognition. Applications reported in the literature includechiral separation of racemic biomolecules, measurement of binding constants, estimation of kinetic on- and off-rate constants, determination of binding stoichiometries (a useful tool in examining electrostaticinteractions), estimation of effective charges and molecular weights of proteins, characterization of enzymatic activities and library screening for tight-binding drug candidates in solution. This technique demands only small amounts of sample (nanolitre injection volumes, picograms of proteins), involves no radiolabelled materials or chemically immobilized ligands, and does not require changes in spectroscopic characteristics upon binding. This paper reviews the most recent applications of affinity capillary electrophoresis and its use in the analysis of biomolecules.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:20:06