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Titolo:
AGE-ASSOCIATED DIFFERENCES IN NEUTROPHIL OXIDATIVE BURST (CHEMILUMINESCENCE)
Autore:
BRAGA PC; SALA MT; DALSASSO M; PECILE A; ANNONI G; VERGANI C;
Indirizzi:
UNIV MILAN,DEPT PHARMACOL,SCH MED,IRCCS,HOSP MAGGIORE,VIA VANVITELLI 32 I-20129 MILAN ITALY UNIV MILAN,SCH MED,IRCCS,MAGGIORE HOSP,DEPT GERIATR I-20129 MILAN ITALY
Titolo Testata:
Experimental gerontology
fascicolo: 5, volume: 33, anno: 1998,
pagine: 477 - 484
SICI:
0531-5565(1998)33:5<477:ADINOB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN POLYMORPHONUCLEAR LEUKOCYTES; RESPIRATORY BURST; CHEMI-LUMINESCENCE; PHAGOCYTOSIS; ACTIVATION; IMMUNITY; OXIDASE;
Keywords:
OXIDATIVE BURST; LUMINOL-AMPLIFIED CHEMILUMINESCENCE; NEUTROPHILS; ELDERLY; CANDIDA ALBICANS; ZYMOSAN; FMLP; PMA; LIQUOID;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
P.C. Braga et al., "AGE-ASSOCIATED DIFFERENCES IN NEUTROPHIL OXIDATIVE BURST (CHEMILUMINESCENCE)", Experimental gerontology, 33(5), 1998, pp. 477-484

Abstract

Phagocytic defensive functions consist of a sequence of events, including migration, phagocytosis, secretion, and the release of reactive oxygen species (ROS). The last of these (also called ''oxidative burst'') has not received due attention in the elderly, even though it can be considered the most important event in the process of killing an invading microorganism. The aim of the present study was to investigate the oxidative burst activity of polymorphonuclear neutrophil leukocytes(PMNs) in relation to age, using a technique that specifically identifies ROS production: luminol-amplified chemiluminescence (LACL). Besides the use of LACL, a particular feature of the study was the use of five rather than just one or two different stimulants: two particulate (Candida albicans and zymosan) and three soluble ones [N-formyl-methionyl-leucyl-phenylalanine (fMLP), phorbol 12 myristate 13 acetate (PMA), and polyanetholesulfonate (liquoid)]. This approach allowed us to observe a dichotomy between the effects of Candida and zymosan (particulates), which were not significantly different in the elderly subjects compared to the young controls, and those of fMLP, PMA, and liquoid (solubles), which showed a significant reduction in LACL in the elderly group. Considering the different results obtained with the various stimulants adopted that are all believed to have NADPH oxidase as a common final target of oxidative burst, it may be postulated that aging caninfluence the different transductional pathways in different ways. (C) 1998 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/02/20 alle ore 03:10:34