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Titolo:
KINETICS AND REGULATION OF FAST ENDOCYTOSIS AT HIPPOCAMPAL SYNAPSES
Autore:
KLINGAUF J; KAVALALI ET; TSIEN RW;
Indirizzi:
STANFORD UNIV,MED CTR,DEPT MOL & CELLULAR PHYSIOL STANFORD CA 94305 STANFORD UNIV,MED CTR,DEPT MOL & CELLULAR PHYSIOL STANFORD CA 94305
Titolo Testata:
Nature
fascicolo: 6693, volume: 394, anno: 1998,
pagine: 581 - 585
SICI:
0028-0836(1998)394:6693<581:KAROFE>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
FROG NEUROMUSCULAR-JUNCTION; SYNAPTIC VESICLE MEMBRANE; CAPACITANCE MEASUREMENTS; TRANSMITTER RELEASE; EXOCYTOSIS; CELLS; CALCIUM; FUSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
30
Recensione:
Indirizzi per estratti:
Citazione:
J. Klingauf et al., "KINETICS AND REGULATION OF FAST ENDOCYTOSIS AT HIPPOCAMPAL SYNAPSES", Nature, 394(6693), 1998, pp. 581-585

Abstract

Presynaptic nerve terminals often contain as few as a hundred vesicles(1,2) and so must recycle them soon after exocytosis to preserve synaptic transmission and presynaptic morphology(3,4) during repetitive firing. The kinetics(3,4) and mechanisms(5) of vesicular endocytosis andrepriming have therefore been studied. Vesicles in hippocampal nerve terminals can become available to release their contents within similar to 40 s of the previous round of exocytosis(6,7). Studies using the styryl dye FM1-43 (ref. 3) have estimated the time constant for endocytosis as similar to 20-30 s (refs 4, 8), at least half of the total recycling time, which is much slower than endocytosis in other secretorysystems(9-11). It seems paradoxical that the neurosecretory terminalsthat could benefit the most from rapid endocytosis do not use such a mechanism. Here we demonstrate the existence of fast endocytosis in hippocampal nerve terminals and derive its kinetics from fluorescence measurements using dyes with varying rates of membrane departitioning. The rapid mode of vesicular retrieval was much faster after exposure tostaurosporine or elevated extracellular calcium. Thus hippocampal synapses take advantage of efficient mechanisms for endocytosis, and their vesicular retrieval is subject to modulatory control.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 24/09/20 alle ore 01:44:39