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Titolo:
PROLACTIN REGULATION OF TISSUE-TYPE PLASMINOGEN-ACTIVATOR AND PLASMINOGEN-ACTIVATOR INHIBITOR TYPE-I GENE-EXPRESSION IN ECG-PRIMED RAT GRANULOSA-CELLS IN CULTURE
Autore:
LIU YX; PENG XR; LIU HZ; CHEN YJ; NY T;
Indirizzi:
CHINESE ACAD SCI,INST ZOOL,STATE KEY LAB REPROD BIOL BEIJING 100080 PEOPLES R CHINA UMEA UNIV,DEPT MED BIOCHEM & BIOPHYS S-90187 UMEA SWEDEN
Titolo Testata:
Biology of reproduction
fascicolo: 2, volume: 59, anno: 1998,
pagine: 409 - 416
SICI:
0006-3363(1998)59:2<409:PROTPA>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONADOTROPIN-RELEASING HORMONE; FOLLICLE-STIMULATING-HORMONE; THECA-INTERSTITIAL CELLS; PREOVULATORY FOLLICLES; PERIOVULATORY PERIOD; INDUCED OVULATION; INVITRO; OVARY; NITROCELLULOSE; COMPLEXES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
51
Recensione:
Indirizzi per estratti:
Citazione:
Y.X. Liu et al., "PROLACTIN REGULATION OF TISSUE-TYPE PLASMINOGEN-ACTIVATOR AND PLASMINOGEN-ACTIVATOR INHIBITOR TYPE-I GENE-EXPRESSION IN ECG-PRIMED RAT GRANULOSA-CELLS IN CULTURE", Biology of reproduction, 59(2), 1998, pp. 409-416

Abstract

The present study was designed to investigate the effect of prolactin(PRL) on plasminogen activator inhibitor-I (PAI-I) and tissue type plasminogen activator (tPA) gene expression in eCG-primed granulosa cells in vitro. At 46 h after the hormone treatment, ovaries were removed,and granulosa cells were prepared for culture. Cells were incubated for various times in serum-free medium in the presence or absence of LHand PRL alone or in combination. tPA and PAI-I activities in the media were assayed by fibrin overlay and reverse fibrin autograph, respectively. Cytoplasmic RNA from granulosa cells was prepared using the NP-40 method and was assayed for PAI-I and tPA mRNA levels. We demonstrated the following. 1) PRL increased PAI-I mRNA production in cultured granulosa cells. Inclusion of LH with PRL had a synergistic effect on increasing PAI-I mRNA levels. After 48-h culture, 3-fold increases in PAI-I mRNA levels were seen with LH in combination with PRL as comparedwith PRL alone. The synergistic increase in PAI-I mRNA levels occurred in a dose- and time-dependent manner. 2) The increase in PAI-I mRNA synthesis by PRL alone, or by PRL in combination with LH, was well correlated with the changes in PAI-I activity and antigen levels in the conditioned media. 3) PRL in the culture also dramatically decreased LH-induced tPA mRNA and activity in a dose- and time-dependent fashion. The decrease in the tPA activity by PRL was also correlated with an increase in the amount of PA-PAI-I complexes in the cell-conditioned media. 4) In situ hybridization of tPA and PAI-I mRNAs in the cultured granulosa cells also showed that PRL was capable of enhancing PAI-I mRNAwhile diminishing tPA mRNA production induced by LH. This suggests that the dose- and time-dependent decrease in the gonadotropin-induced tPA activity in the culture by the presence of PRL may be due to decreasing tPA mRNA synthesis on one hand and to neutralization of the tPA activity by the increased PAI-I activity on the other.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/09/20 alle ore 21:42:01