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Titolo:
CHARACTERIZATION OF THE IMMOBILIZED BETA-GALACTOSIDASE-C FROM BACILLUS-CIRCULANS AND THE PRODUCTION OF BETA(1-]3)-LINKED DISACCHARIDES
Autore:
NAUNDORF A; CAUSSETTE M; AJISAKA K;
Indirizzi:
MEIJI MILK PROD CO LTD,INST HLTH SCI,540 NARUDA ODAWARA KANAGAWA 250086 JAPAN MEIJI MILK PROD CO LTD,INST HLTH SCI ODAWARA KANAGAWA 250086 JAPAN
Titolo Testata:
Bioscience, biotechnology, and biochemistry
fascicolo: 7, volume: 62, anno: 1998,
pagine: 1313 - 1317
SICI:
0916-8451(1998)62:7<1313:COTIBF>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOSYLATION; DERIVATIVES; INHIBITION; ENZYMES;
Keywords:
BETA-GALACTOSIDASE; BACILLUS CIRCULANS; IMMOBILIZATION; TRANSGLYCOSYLATION; GAL-BETA(1-]3)-GALNAC;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
21
Recensione:
Indirizzi per estratti:
Citazione:
A. Naundorf et al., "CHARACTERIZATION OF THE IMMOBILIZED BETA-GALACTOSIDASE-C FROM BACILLUS-CIRCULANS AND THE PRODUCTION OF BETA(1-]3)-LINKED DISACCHARIDES", Bioscience, biotechnology, and biochemistry, 62(7), 1998, pp. 1313-1317

Abstract

A recombinant beta-galactosidase, which was obtained from the beta-galactosidase C gene of Bacillus circulans and cleaves the non-reducing end galactosyl residue of beta(1-->3)-linkages selectively, was immobilized using CNBr-Sepharose. Although the effect of pH was not changed by the immobilization, the thermostability and stability in the presence of DMF were increased. Optimization of the transglycosylation usingpara-nitrophenyl beta-D-galactopyranoside as a donor and benzyl-alpha-D-N-acetylgalactosaminide as an acceptor afforded a beta(1-->3)-linked disaccharide derivative with 62% molar yield in a gram scale synthesis. Using the methyl-analogue as an acceptor, 53% of the acceptor was converted to the respective beta(1-->3)-disaccharide.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/09/20 alle ore 03:11:27