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Titolo:
OXYGEN-TENSION ALTERS THE EFFECTS OF CYTOKINES ON THE MEGAKARYOCYTE, ERYTHROCYTE, AND GRANULOCYTE LINEAGES
Autore:
LAIUPPA JA; PAPOUTSAKIS ET; MILLER WM;
Indirizzi:
NORTHWESTERN UNIV,DEPT CHEM ENGN EVANSTON IL 60208 NORTHWESTERN UNIV,DEPT CHEM ENGN EVANSTON IL 60208
Titolo Testata:
Experimental hematology
fascicolo: 9, volume: 26, anno: 1998,
pagine: 835 - 843
SICI:
0301-472X(1998)26:9<835:OATEOC>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
HEMATOPOIETIC GROWTH-FACTORS; COLONY-STIMULATING FACTOR; HUMAN BONE-MARROW; HUMAN CORD BLOOD; C-MPL LIGAND; PROGENITOR CELLS; PROLIFERATION INVITRO; STEM-CELLS; IN-VITRO; ERYTHROPOIETIN;
Keywords:
OXYGEN TENSION; CYTOKINES; PERIPHERAL BLOOD; MEGAKARYOCYTES; GRANULOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
44
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Laiuppa et al., "OXYGEN-TENSION ALTERS THE EFFECTS OF CYTOKINES ON THE MEGAKARYOCYTE, ERYTHROCYTE, AND GRANULOCYTE LINEAGES", Experimental hematology, 26(9), 1998, pp. 835-843

Abstract

Many investigators believe that cytokines have exclusive control overproliferation and differentiation in stroma-free hematopoietic cultures. Although cytokines are indeed necessary, other culture parameters such as oxygen (O-2) tension can greatly influence both hematopoietic cell proliferation and differentiation. We investigated the effects ofcytokine combinations and O-2 tension on the expansion of megakaryocyte (size and number of CD41a(+) cells and number of colony-forming units-megakaryocyte [CFU-Mk]), granulocyte (CD15(+) cells and CFU-granulocyte monocyte [CFU-GM]), and erythrocyte (hemoglobin(+) cells and burst-forming units-erythrocyte [BFU-E]) lineages. Peripheral blood CD34(+) cells were cultured in serum-free medium with interleukin (IL)-3, stem cell factor (SCF), and various combinations of thrombopoietin (TPO), erythropoietin (EPO), and/or granulocyte-colony stimulating factor (G-CSF). The effects of TPO, EPO, and G-CSF on Mks, erythrocytes, and granulocytes, respectively, were dependent on the O-2 tension. Thrombopoietin-containing cultures under a gas phase of 20% O-2 tension produced 1.4- to 2.2-fold more Mks than those under 5% O-2. The increase in Mk size with TPO was also much greater under 20% O-2 Similarly, 2.1- to 2.4-fold more hemoglobin-containing cells were produced in EPO-containing cultures under 20% vs. 5% O-2. In contrast, approximately twice as many CD15(+) cells were produced in G-CSF-containing cultures under5% vs. 20% O-2. The numbers of CFU-Mk in TPO-containing and CFU-GM inG-CSF-containing cultures were larger under 5% O-2. Although the O-2 tension had no effect on BFU-E production in EPO-containing cultures, BFU-E production under 5% O-2 in cultures without EPO was equal to that in EPO-containing cultures. Our data also suggest that TPO, EPO, andG-CSF elicit stimulatory cross-lineage effects in the presence of IL-3 and SCF, and that these effects, too, are often dependent on O-2 tension.

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Documento generato il 11/07/20 alle ore 14:37:16