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Titolo:
MULTIPLE BINDING-SITES FOR [I-125] RTI-121 AND OTHER COCAINE ANALOGS IN RAT FRONTAL CEREBRAL-CORTEX
Autore:
BOJA JW; CARROLL FI; VAUGHAN RA; KOPAJTIC T; KUHAR MJ;
Indirizzi:
NE OHIO UNIV,COLL MED,DEPT PHARMACOL,4209 SR 44,POB 95 ROOTSTOWN OH 44266 RES TRIANGLE INST RES TRIANGLE PK NC 27709 NIDA,INTRAMURAL RES PROGRAM,NEUROSCI BRANCH,NIH BALTIMORE MD 00000 EMORY UNIV,YERKES REG PRIMATE RES CTR ATLANTA GA 30322
Titolo Testata:
Synapse
fascicolo: 1, volume: 30, anno: 1998,
pagine: 9 - 17
SICI:
0887-4476(1998)30:1<9:MBF[RA>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
MEDIAL PREFRONTAL CORTEX; H-3 GBR-12935 BINDING; HUMAN DOPAMINE TRANSPORTER; NUCLEUS-ACCUMBENS; REINFORCING PROPERTIES; STRIATAL MEMBRANES; BRAIN SYNAPTOSOMES; SELF-STIMULATION; GLYCOSYLATION; LOCALIZATION;
Keywords:
COCAINE; RTI-121; DOPAMINE TRANSPORTER; SEROTONIN TRANSPORTER; NOREPINEPHRINE TRANSPORTER;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
J.W. Boja et al., "MULTIPLE BINDING-SITES FOR [I-125] RTI-121 AND OTHER COCAINE ANALOGS IN RAT FRONTAL CEREBRAL-CORTEX", Synapse, 30(1), 1998, pp. 9-17

Abstract

In an effort to identify novel binding sites for cocaine and its analogs, we carried out binding studies with the high-affinity and selective ligand [I-125]RTI-121 in rat frontal cortical tissue. Very low densities of binding sites were found. Saturation analysis revealed that the binding was to both high- and low-affinity sites. Pharmacological competition studies were carried out with inhibitors of the dopamine, norepinephrine, and serotonin transporters. The various transporter inhibitors inhibited the binding of 15 pM [I-125]RTI-121 in a biphasic fashion following a two-site binding model. The resultant data were complex and did not suggest a simple association with any single transporter. Correlational analysis supported the following hypothesis: [I-125]RTI-121 binds to known transporters and not to novel sites; these include dopamine, norepinephrine, and serotonin transporters. Immunoprecipitation of transporters photoaffinity labeled with [(125)] RTI-82 andsubsequent analysis of SDS-page gels revealed the presence of authentic dopamine transporters in these samples; displacement of the photoaffinity label occurred with a typical dopamine transporter pharmacology. These data are compatible with the binding properties of RTI-181 andthe presence of several known transporters in the tissue studied. Synapse 30:9-17, 1998. (C) 1998 Wiley-Liss, Inc.dagger

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 13/07/20 alle ore 18:02:04