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Titolo:
EXPRESSION OF INTERLEUKIN-1-BETA MESSENGER-RNA AND PROTEIN IN HUMAN GLIOMAS ASSESSED BY RT-PCR AND IMMUNOHISTOCHEMISTRY
Autore:
SASAKI A; TAMURA M; HASEGAWA M; ISHIUCHI S; HIRATO J; NAKAZATO Y;
Indirizzi:
GUNMA UNIV,SCH MED,DEPT PATHOL,3-39-22 SHOWA MACHI MAEBASHI GUMMA 3718511 JAPAN GUNMA UNIV,SCH MED,DEPT NEUROSURG MAEBASHI GUMMA 3718511 JAPAN GUNMA UNIV,SCH MED,DEPT RADIOL & RADIAT ONCOL MAEBASHI GUMMA 3718511 JAPAN ISESAKI CITY HOSP ISESAKI 3720812 JAPAN
Titolo Testata:
Journal of neuropathology and experimental neurology
fascicolo: 7, volume: 57, anno: 1998,
pagine: 653 - 663
SICI:
0022-3069(1998)57:7<653:EOIMAP>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; MESSENGER-RNA EXPRESSION; HUMAN GLIOBLASTOMA CELLS; ASTROGLIAL PROLIFERATION; CONVERTING-ENZYME; MONONUCLEAR-CELLS; MALIGNANT GLIOMAS; BRAIN-TUMORS; IN-VITRO; INDUCTION;
Keywords:
CYTOKINE; GLIOMA; INTERLEUKIN-1; MACROPHAGE; MICROGLIA; NUDE MICE; POLYMERASE CHAIN REACTION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
49
Recensione:
Indirizzi per estratti:
Citazione:
A. Sasaki et al., "EXPRESSION OF INTERLEUKIN-1-BETA MESSENGER-RNA AND PROTEIN IN HUMAN GLIOMAS ASSESSED BY RT-PCR AND IMMUNOHISTOCHEMISTRY", Journal of neuropathology and experimental neurology, 57(7), 1998, pp. 653-663

Abstract

To characterize the expression and localization of interleukin (IL)-1beta in human gliomas, both reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistochemistry were used on surgically excised human,gliomas, human malignant glioma xenografts, and human glioblastoma cell lines. The RT-PCR products for IL-1 beta mRNA were quantified by computerized image analysis. IL-1 beta mRNA was detectable in 30out of 35 (86%) surgically resected gliomas. An abundant expression of IL-1 beta mRNA was often found in the glioblastomas, anaplastic astrocytomas, and pilocytic astrocytomas, but not in other types of gliomas. Quantitatively, in both the grade 2 astrocytomas and the oligodendrogliomas, the IL-1 beta mRNA levels were significantly (p < 0.05) lower than those of the grade 3/4 astrocytomas. Immunohistochemically, IL-1 beta was localized in the pleomorphic tumor cells of the astrocytic tumors and in macrophages. In contrast to the astrocytic tumors, low and high grade oligodendrogliomas showed no or little expression of IL-1 beta antigen, IL-1 beta was present less frequently than IL-1 alpha and IL-1 receptor type 1 in 3 malignant gliomas transplanted into nudemice by RT-PCR. All 2 cell lines showed IL-1 beta expression at both the mRNA and protein levels. It is concluded that in human gliomas, both high-grade astrocytomas and pilocytic astrocytomas often express high IL-1 beta production, and that IL-1 beta is mainly localized in astrocytic tumor cells and macrophages.

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Documento generato il 20/09/20 alle ore 07:22:27