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Titolo:
THE CRYSTAL-STRUCTURE OF FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS-JANNASCHII
Autore:
HWANG KY; BAEK K; KIM HY; CHO Y;
Indirizzi:
KOREA ADV INST SCI & TECHNOL,STRUCT BIOL CTR,POB 13 SEOUL 130650 SOUTH KOREA
Titolo Testata:
Nature structural biology
fascicolo: 8, volume: 5, anno: 1998,
pagine: 707 - 713
SICI:
1072-8368(1998)5:8<707:TCOFEF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
COLI DNA-POLYMERASE; EXONUCLEASE; REPLICATION; REPAIR; 5'-EXONUCLEASE; CLEAVAGE; SEQUENCE; HOMOLOGY; DOMAIN; FEN-1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
35
Recensione:
Indirizzi per estratti:
Citazione:
K.Y. Hwang et al., "THE CRYSTAL-STRUCTURE OF FLAP ENDONUCLEASE-1 FROM METHANOCOCCUS-JANNASCHII", Nature structural biology, 5(8), 1998, pp. 707-713

Abstract

Flap endonuclease-1 (FEN-1), a structure specific nuclease, is an essential enzyme for eukaryotic DNA replication and repair. The crystal structure of FEN-1 from Methanococcus jannaschii, determined at 2.0 Angstrom resolution, reveals an active site with two metal ions residing on top of a deep cleft where several conserved acidic residues are clustered. Near the active site, a long flexible loop comprised of many basic and aromatic residues forms a hole large enough to accommodate the DNA substrate. Deletion mutations in this loop significantly decreased the nuclease activity and specificity of FEN-1, suggesting that theloop is critical for recognition and cleavage of the junction betweensingle and double-stranded regions of flap DNA.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 10/07/20 alle ore 14:52:33