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Titolo:
IMPROVED ISOTOPIC ABUNDANCE MEASUREMENTS FOR HIGH-RESOLUTION FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTRA VIA TIME-DOMAIN DATA EXTRACTION
Autore:
BRESSON JA; ANDERSON GA; BRUCE JE; SMITH RD;
Indirizzi:
BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM & MOL SCI LAB,POB 999 RICHLAND WA 99352 BATTELLE MEM INST,PACIFIC NW LABS,ENVIRONM & MOL SCI LAB RICHLAND WA 99352
Titolo Testata:
Journal of the American Society for Mass Spectrometry
fascicolo: 8, volume: 9, anno: 1998,
pagine: 799 - 804
SICI:
1044-0305(1998)9:8<799:IIAMFH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
FREQUENCY-SHIFTS; INDIVIDUAL IONS; SPECTROMETRY; DISTRIBUTIONS; BIOMOLECULES; MOLECULES; CHEMISTRY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
26
Recensione:
Indirizzi per estratti:
Citazione:
J.A. Bresson et al., "IMPROVED ISOTOPIC ABUNDANCE MEASUREMENTS FOR HIGH-RESOLUTION FOURIER-TRANSFORM ION-CYCLOTRON RESONANCE MASS-SPECTRA VIA TIME-DOMAIN DATA EXTRACTION", Journal of the American Society for Mass Spectrometry, 9(8), 1998, pp. 799-804

Abstract

The simultaneous high resolution and accurate mass measurements possible with Fourier transform ion cyclotron resonance mass spectrometry coupled with the gentle ionization of electrospray hold attractions forprotein, peptide, and oligonucleotide characterization, including multistage-mass spectrometry measurements for assignment of fragment masses and greater confidence in structural measurements. The detection ofcyclotron motion over extended periods of time (in some cases for several minutes) allows higher resolution and mass accuracy. Generally, signal duration has been considered to be limited primarily by background pressure, with ion-neutral collisions leading to the reduction and dephasing of cyclotron motion, causing signal loss. However, recent theoretical work has shown that the ion cloud stability that is a prerequisite for high performance measurements is highly dependent on the electric field generated by the ion cloud, thus giving rise to a minimumnumber of charges or ions required for extended time-domain signals. The effects of ion population on ion cloud stability and signal duration, and the subsequent effects on resolution and measured isotopic abundances are reported. Individual time-domain signals for bovine insulin isotopic peaks were extracted to allow a comparison of the damping rates for each of these ion clouds and the measured time-domain amplitude maxima are shown to provide a better match with the theoretically predicted isotopic abundances for insulin. These results show that different damping rates of ions of very similar mass, but different ion cloud population sizes, can have dramatic effects on the observed isotopic patterns. Additionally, more accurate, high resolution spectra can be produced by correcting for the effects of the different damping rates that are observed for different ion population sizes. (C) 1998 American Society for Mass Spectrometry.

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Documento generato il 05/07/20 alle ore 13:34:31