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Titolo:
A RAPID-DETERMINATION OF ALLANTOIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING TRIS(HYDROXYMETHYL)AMINOMETHANE-HCL BUFFER AS A MOBILE-PHASE
Autore:
YAMAMOTO R; SEKI M; OUCHI K; KOYANO S; NAKAZAWA S; NAGATANI Y; SATO H;
Indirizzi:
ZERIA PHARMACEUT CO LTD,CENT RES LABS,2512-1 OSHIKIRI KONAN SAITAMA 3600111 JAPAN ZERIA PHARMACEUT CO LTD,CENT RES LABS KONAN SAITAMA 3600111 JAPAN
Titolo Testata:
Yakugaku zasshi
fascicolo: 8, volume: 118, anno: 1998,
pagine: 310 - 316
SICI:
0031-6903(1998)118:8<310:AROABH>2.0.ZU;2-V
Fonte:
ISI
Lingua:
JPN
Soggetto:
BIOLOGICAL-FLUIDS;
Keywords:
ALLANTOIN; HPLC; ODS COLUMN; TRIS; ION-PAIR REAGENT; EYEDROP;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
10
Recensione:
Indirizzi per estratti:
Citazione:
R. Yamamoto et al., "A RAPID-DETERMINATION OF ALLANTOIN BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY USING TRIS(HYDROXYMETHYL)AMINOMETHANE-HCL BUFFER AS A MOBILE-PHASE", Yakugaku zasshi, 118(8), 1998, pp. 310-316

Abstract

A rapid and simple method for the determination of allantoin in pharmaceuticals by reversed-phase ion-pair high-performance liquid chromatography using an ODS column was presented. In general, it is difficult to retain allantoin to the ODS column owing to its very low hydrophobicity. We solved these problems by the use of a Tris-HCl buffer (pH 7.5) containing tetra-n-hexyl-ammonium bromide (THAB) as an ion-pair reagent for the mobile phase. Comparatively low concentrations of Tris-HClbuffer (0.9 mM) and THAB (0.5 mM) gave a high capacity factor (k'). As a results of the er;amination of the chromatographic behavior, it isconfirmed that the retention mechanism of allantoin to the ODS columnon the present method was not the ion-pair mode, but the ion-exchangemade. Calibration curves for allantoin showed a good linearity in therange of 10 to 400 mu g/ml (r = 0.9999). The reproducibility (R.S.D.,n = 6) was invariably good (0.37%). The lowest concentration of allantoin for the determination was 200 nb per 20 mu l of injection. The present method was successfully applied to the determination of allantoin in commercial eyedrops with good recovery (99.4%). It was found thatallantoin in pharmaceuticals could be determined by the present method in short time and without any complicated derivatization.

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Documento generato il 03/07/20 alle ore 01:24:09