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Titolo:
STRETCH-ACTIVATED CATION CHANNEL IN HUMAN UMBILICAL VEIN ENDOTHELIUM IN NORMAL-PREGNANCY AND IN PREECLAMPSIA
Autore:
KOHLER R; SCHONFELDER G; HOPP H; DISTLER A; HOYER J;
Indirizzi:
FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,DEPT NEPHROL,HINDENBURGDAMM 30 D-12200 BERLIN GERMANY FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,DEPT INTERNAL MED & NEPHROL D-12200 BERLIN GERMANY FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,DEPT CLIN PHARMACOL & TOXICOL D-12200 BERLIN GERMANY FREE UNIV BERLIN,KLINIKUM BENJAMIN FRANKLIN,DEPT OBSTET & GYNECOL D-12200 BERLIN GERMANY
Titolo Testata:
Journal of hypertension
fascicolo: 8, volume: 16, anno: 1998,
pagine: 1149 - 1156
SICI:
0263-6352(1998)16:8<1149:SCCIHU>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
ION CHANNELS; RELAXING FACTOR; PATCH-CLAMP; CELLS; FLOW; CALCIUM; MEMBRANE; RELEASE; MUSCLE; INFLUX;
Keywords:
ION CHANNELS; MECHANOTRANSDUCTION; ENDOTHELIUM; HUMAN UMBILICAL VEIN; PREECLAMPSIA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
29
Recensione:
Indirizzi per estratti:
Citazione:
R. Kohler et al., "STRETCH-ACTIVATED CATION CHANNEL IN HUMAN UMBILICAL VEIN ENDOTHELIUM IN NORMAL-PREGNANCY AND IN PREECLAMPSIA", Journal of hypertension, 16(8), 1998, pp. 1149-1156

Abstract

Objective. To determine whether stretch-activated cation channels (SAC) are present in intact human umbilical vein endothelium (HUVE) and in an endothelial cell line (EA.hy) and whether they act as endothelialmechanosensors, and to determine whether endothelial SAC in HUVE fromwomen with pregnancies complicated by preeclampsia undergo functionalchanges compared with those in HUVE from women with normotensive pregnancies. Methods and results. By use of the patch-clamp technique we identified a SAC in intact HUVE and in an endothelial cell line, The SAC had mean conductances of 29 +/- 5 pS (n = 38) for K+ and 12 +/- 2 pS(n = 4) for Ca2+, Administration of 50 mu mol/l gadolinium, a blockerof mechanosensitive ion channels, completely blocked activity of thischannel. We found from single-channel recordings that influx of Ca2+ through SAC directly activated high-conductance Ca2+-dependent potassium channels, proving that a significant influx of Ca2+ through SAC occurs at physiologic concentrations of Ca2+. In a comparative study, apparent channel density of SAC (percentage of patches with SAC activity)in HUVE from women with pregnancies complicated by preeclampsia (36.2+/- 4.3%) was twofold higher than that in HUVE from women with normalpregnancies (17.9 +/- 2.9%, P < 0.01). Channel conductance and sensitivity to stretching of SAC were not altered by preeclampsia. Conclusions. Since SAC are capable of acting as endothelial mechanosensors, thegreater than normal density of SAC associated with preeclampsia mightreflect an alteration of mechanotransduction. J Hypertens 16:1149-1156. (C) 1998 Lippincott-Raven Publishers.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/10/20 alle ore 03:24:26