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Titolo:
ENHANCED EXPRESSION OF HEAT-SHOCK-PROTEIN-70 (HSP70) AND HEAT-SHOCK FACTOR-1 (HSF1) ACTIVATION IN RHEUMATOID-ARTHRITIS SYNOVIAL TISSUE - DIFFERENTIAL REGULATION OF HSP70 EXPRESSION AND HSF1 ACTIVATION IN SYNOVIAL FIBROBLASTS BY PROINFLAMMATORY CYTOKINES, SHEAR-STRESS, AND ANTIINFLAMMATORY DRUGS
Autore:
SCHETT G; REDLICH K; XU QB; BIZAN P; GROGER M; TOHIDASTAKRAD M; KIENER H; SMOLEN J; STEINER G;
Indirizzi:
UNIV VIENNA,DEPT INTERNAL MED 3,DIV RHEUMATOL,WAHRINGER GURTEL 18-20 A-1090 VIENNA AUSTRIA UNIV VIENNA,DEPT INTERNAL MED 3,DIV RHEUMATOL A-1090 VIENNA AUSTRIA AUSTRIAN ACAD SCI,INST BIOMED AGING RES INNSBRUCK AUSTRIA UNIV VIENNA,CLIN ORTHOPED VIENNA AUSTRIA UNIV VIENNA,DEPT IMMUNODERMATOL VIENNA AUSTRIA LUDWIG BOLTZMANN INST RHEUMATOL & BALNEOL VIENNA AUSTRIA
Titolo Testata:
The Journal of clinical investigation
fascicolo: 2, volume: 102, anno: 1998,
pagine: 302 - 311
SICI:
0021-9738(1998)102:2<302:EEOH(A>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
TUMOR-NECROSIS-FACTOR; SYSTEMIC LUPUS-ERYTHEMATOSUS; JUVENILE CHRONIC ARTHRITIS; SODIUM-SALICYLATE; GENE-TRANSCRIPTION; FACTOR-ALPHA; ADHESION MOLECULE-1; MONONUCLEAR-CELLS; AUTOANTIBODIES; CARTILAGE;
Keywords:
STRESS PROTEINS; FIBROBLASTS; SYNOVIAL CELLS; ARTHRITIS; TUMOR NECROSIS FACTOR-ALPHA;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
60
Recensione:
Indirizzi per estratti:
Citazione:
G. Schett et al., "ENHANCED EXPRESSION OF HEAT-SHOCK-PROTEIN-70 (HSP70) AND HEAT-SHOCK FACTOR-1 (HSF1) ACTIVATION IN RHEUMATOID-ARTHRITIS SYNOVIAL TISSUE - DIFFERENTIAL REGULATION OF HSP70 EXPRESSION AND HSF1 ACTIVATION IN SYNOVIAL FIBROBLASTS BY PROINFLAMMATORY CYTOKINES, SHEAR-STRESS, AND ANTIINFLAMMATORY DRUGS", The Journal of clinical investigation, 102(2), 1998, pp. 302-311

Abstract

shock proteins (hsp) have been repeatedly implicated to participate in the pathogenesis of rheumatoid arthritis (RA), Herein, we investigated the regulation of synovial hsp70 expression by analyzing the DNA-binding activity of heat shock transcription factor 1 (HSF1) as well as inducible hsp70 expression, Experiments were performed both on synovial tissue and on synovial fibroblast-like cells (SFC). Gel mobility shift analysis revealed increased HSF1 activation, and Western blotting and immunohistochemistry revealed increased hsp70 expression in RA synovial tissue, but not in synovial tissue derived from patients with osteoarthritis, Proinflammatory cytokines (TNF-alpha, IL-1 alpha, IL-6), but not IFN-gamma or TGF-beta, induced activation of HSF1-DNA binding and hsp70 expression in cultivated SFC, Activation of HSF1 in SFC was accompanied by hyperphosphorylation and nuclear translocation of HSF1,Furthermore, shear stress also induced a complete heat shock responsein cultivated synovial cells, In contrast, nonsteroidal antiinflammatory drugs triggered only an incomplete heat shock response, with HSF1 activation but not hsp70 induction, whereas steroids and immunosuppressive drugs did not affect the heat shock response at all, In summary, these data suggest that induction of hsp70 expression in rheumatoid synovial tissue is based on transcriptional activation of HSF1 due to the presence of proinflammatory cytokines (and possibly also shear stress).

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 21:53:51