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Titolo:
DIFFERENT REGIONS OF RHO DETERMINE RHO-SELECTIVE BINDING OF DIFFERENTCLASSES OF RHO-TARGET MOLECULES
Autore:
FUJISAWA K; MADAULE P; ISHIZAKI T; WATANABE G; BITO H; SAITO Y; HALL A; NARUMIYA S;
Indirizzi:
KYOTO UNIV,FAC MED,DEPT PHARMACOL,SAKYO KU KYOTO 6068315 JAPAN UNIV LONDON UNIV COLL,MRC,MOL CELL BIOL LAB,CRC ONCOGENE & SIGNAL TRANSDUCT GRP LONDON WC1E 6BT ENGLAND UNIV LONDON UNIV COLL,DEPT BIOCHEM LONDON WC1E 6BT ENGLAND KYOTO UNIV,FAC MED,DEPT PHARMACOL,SAKYO KU KYOTO 606 JAPAN
Titolo Testata:
The Journal of biological chemistry
fascicolo: 30, volume: 273, anno: 1998,
pagine: 18943 - 18949
SICI:
0021-9258(1998)273:30<18943:DRORDR>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
H-RAS P21; ADP-RIBOSYLATION; PROTEIN-RHO; SERINE/THREONINE KINASE; GENE-PRODUCT; EFFECTOR REGION; COILED-COIL; DOMAIN; GTPASE; ACTIVATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
45
Recensione:
Indirizzi per estratti:
Citazione:
K. Fujisawa et al., "DIFFERENT REGIONS OF RHO DETERMINE RHO-SELECTIVE BINDING OF DIFFERENTCLASSES OF RHO-TARGET MOLECULES", The Journal of biological chemistry, 273(30), 1998, pp. 18943-18949

Abstract

Based on their Rho binding motifs several Rho target molecules can beclassified into three groups; class I includes the protein kinase PKN, rhophilin, and rhotekin, class II includes the protein kinases, Rho-associated coiled-coil containing protein kinases, ROCK-I and ROCK-II,and class III includes citron, Taking advantage of the selectivity inrecognition by these targets between Rho and Rac, we examined the regions in Rho required for selective binding of each class of Rho targetmolecules, Yeast two-hybrid assays were performed using Rho/Rac chimeras and either rhophilin, ROCK-I, or citron, This study showed the existence of act least two distinct regions in Rho (amino acids 23-40 and75-92) that are critical for the selective binding of these targets. The former was required for binding: to citron, whereas the latter wasnecessary for binding to rhophilin, On the other hand, either region showed affinity to ROCK-I. This was further confirmed by ligand overlay assay using both recombinant ROCK-I and ROCK-II proteins. Consistently, Rho/Rac chimeras containing either region can induce stress fibersin transfected HeLa cells, and this induction is suppressed by treatment with Y-27632, a specific inhibitor of ROCK kinases. These results suggest that the selective binding of different classes of Rho targetsto Rho is determined by interaction between distinct Rho-binding motifs of the targets and different regions of Rho.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 21:18:33