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Titolo:
COMPARATIVE GENOMIC HYBRIDIZATION WITH LISSAMINE-LABELED AND FLUORESCEIN-LABELED NUCLEOTIDES
Autore:
ROSENBERG C; VANGILSWIJK RPM; VOS CBJ; WIEGANT J; CORNELISSE CJ; TANKE HJ; RAAP AK;
Indirizzi:
LEIDEN UNIV,MED CTR,DEPT MOL CELL BIOL,LAB CYTOCHEM & CYTOMETRY NL-2333 AL LEIDEN NETHERLANDS UNIV ROTTERDAM HOSP,DR DANIEL DEN HOED CANC CTR,LAB EXPT PATHOONCOL ROTTERDAM NETHERLANDS LEIDEN UNIV,MED CTR,DEPT PATHOL LEIDEN NETHERLANDS
Titolo Testata:
Cytometry
fascicolo: 4, volume: 32, anno: 1998,
pagine: 337 - 341
SICI:
0196-4763(1998)32:4<337:CGHWLA>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
SEQUENCE COPY NUMBER; INSITU HYBRIDIZATION; QUANTITATIVE-ANALYSIS; SOLID TUMORS; DNA; AMPLIFICATION; GAINS; IDENTIFICATION; LOSSES; CANCER;
Keywords:
CGH; DNA LABELING; FLUOROCHROMES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
25
Recensione:
Indirizzi per estratti:
Citazione:
C. Rosenberg et al., "COMPARATIVE GENOMIC HYBRIDIZATION WITH LISSAMINE-LABELED AND FLUORESCEIN-LABELED NUCLEOTIDES", Cytometry, 32(4), 1998, pp. 337-341

Abstract

Biotin deoxyuridine 5'-triphosphate (dUTP) and digoxigenin dUTP are the labels most commonly used in comparative genomic hybridization (CGH). The relative infrequent use of direct fluorochrome-labeled nucleotides in CGH is related to the lower sensitivity they provide. Here we report the evaluation of two fluorochrome-conjugated nucleotides that have not been previously used in CGH (lissamine-5-dUTP and fluorescein-N-6-dATP) and show that this direct label combination performs at least as well as the indirect biotin/digoxigenin pair. Cytometry 32:337-341, 1998, (C) 1998 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/11/20 alle ore 15:47:26