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Titolo:
GENE-EXPRESSION OF DIFFERENTIATION-SPECIFIC KERATIN-4 13 AND KERATIN-1/10 IN NORMAL HUMAN BUCCAL MUCOSA/
Autore:
BLOOR BK; SU L; SHIRLAW PJ; MORGAN PR;
Indirizzi:
UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,GUYS HOSP,DEPT ORAL MED &PATHOL LONDON SE1 9RT ENGLAND UNITED MED & DENT SCH GUYS & ST THOMAS HOSP,GUYS HOSP,DEPT ORAL MED &PATHOL LONDON SE1 9RT ENGLAND UNIV LOUISVILLE,SCH DENT,DEPT BIOL BIOPHYS SCI LOUISVILLE KY 40292
Titolo Testata:
Laboratory investigation
fascicolo: 7, volume: 78, anno: 1998,
pagine: 787 - 795
SICI:
0023-6837(1998)78:7<787:GODK1A>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
CERVICAL INTRAEPITHELIAL NEOPLASIA; MONOCLONAL-ANTIBODIES; MESSENGER-RNA; CYTOKERATIN GENE; EPITHELIA; EPIDERMIS; TUMORS; PATTERNS; SKIN; HYPERPROLIFERATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
34
Recensione:
Indirizzi per estratti:
Citazione:
B.K. Bloor et al., "GENE-EXPRESSION OF DIFFERENTIATION-SPECIFIC KERATIN-4 13 AND KERATIN-1/10 IN NORMAL HUMAN BUCCAL MUCOSA/", Laboratory investigation, 78(7), 1998, pp. 787-795

Abstract

The aim of the present study was to compare gene expression of the major differentiation-specific keratins in oral epithelium: keratins 4, 13, 1, and 10. Previous studies have shown that the dominant keratins in normal buccal epithelium are K4 and K13, with minor populations of cells showing K1 and K10 expression; herein, we have further examined expression of these keratins at the mRNA level. Six biopsies from normal buccal mucosa were immunohistochemically stained for keratin proteins by means of monoclonal antibodies to K4, K13, K1, and K10. Adjacentsections were processed for mRNA by nonisotopic in situ hybridization, using specific riboprobes labeled with digoxigenin. Proteins for K4 and K13 were expressed suprabasally throughout buccal epithelium, withcolumns of cells staining additionally for K1 and K10. In situ hybridization revealed a comparable pattern of mRNA distribution for K4 and K13, with expression restricted to parabasal and lower prickle cells. Transcripts for K1 and K10 were present in basal, parabasal, and lowerprickle layers, showing a much wider expression than that of their proteins. This study has shown that in buccal epithelium, there is extensive mRNA expression of the ''inappropriate'' differentiation-specifickeratins, despite minimal protein expression. This suggests that K1 and K10 are regulated at the post-transcriptional level, so that they may be expressed adaptively as proteins. The findings will form a useful baseline for the study of these keratins in pathologically altered oral epithelia as well as in nonkeratinized epithelia from extra-oral sites.

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Documento generato il 21/09/20 alle ore 12:42:34