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Titolo:
AN ESSENTIAL ROLE FOR A SMALL SYNAPTIC VESICLE-ASSOCIATED PHOSPHATIDYLINOSITOL 4-KINASE IN NEUROTRANSMITTER RELEASE
Autore:
WIEDEMANN C; SCHAFER T; BURGER MM; SIHRA TS;
Indirizzi:
UNIV COLL LONDON,DEPT PHARMACOL,MEDAWAR BLDG,GOWER ST LONDON WC1E 6BTENGLAND UNIV LONDON,ROYAL FREE HOSP,SCH MED,DEPT PHARMACOL LONDON NW3 2PF ENGLAND FRIEDRICH MIESCHER INST CH-4002 BASEL SWITZERLAND
Titolo Testata:
The Journal of neuroscience
fascicolo: 15, volume: 18, anno: 1998,
pagine: 5594 - 5602
SICI:
0270-6474(1998)18:15<5594:AERFAS>2.0.ZU;2-G
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROTEIN-KINASE-C; ADP-RIBOSYLATION FACTOR; GLUTAMATE RELEASE; CHROMAFFIN CELLS; PHOSPHATIDIC-ACID; PHOSPHOLIPASE-D; CEREBROCORTICAL SYNAPTOSOMES; CA2+-ACTIVATED SECRETION; ENDOCRINE-CELLS; NERVE-TERMINALS;
Keywords:
PHOSPHOINOSITIDES; GLUTAMATE EXOCYTOSIS; SYNAPTOSOMES; SMALL SYNAPTIC VESICLES; PHOSPHOLIPIDS; LIPID KINASE; PHOSPHATIDYLINOSITOL 4-KINASE; PRIMING; ATP DEPENDENT; CALCIUM DEPENDENT; KINASE INHIBITORS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
61
Recensione:
Indirizzi per estratti:
Citazione:
C. Wiedemann et al., "AN ESSENTIAL ROLE FOR A SMALL SYNAPTIC VESICLE-ASSOCIATED PHOSPHATIDYLINOSITOL 4-KINASE IN NEUROTRANSMITTER RELEASE", The Journal of neuroscience, 18(15), 1998, pp. 5594-5602

Abstract

Glutamate release from nerve terminals is the consequence of Ca2+-triggered fusion of small synaptic vesicles with the presynaptic plasma membrane. ATP dependence of neurotransmitter release has been suggestedto be founded, in part, on phosphorytation steps preceding membrane fusion. Here we present evidence for an essential role of phosphatidylinositol phosphorylation in stimulated release of neurotransmitter glutamate from isolated nerve terminals (synaptosomes). Specifically, we show that a phosphatidylinositol 4-kinase (PtdIns 4-kinase) activity resides on nerve terminal-derived small synaptic Vesicles (SSVs) and that inhibition of the PtdIns 4-kinase activity in intact synaptosomes leads to attenuation of the evoked release of glutamate. The attenuationof transmitter release is reversible and correlates with respective changes in intrasynaptosomal PtdIns 4-kinase activity Because only the Ca2+-dependent release of glutamate is affected, regulation appears tobe at the level of exocytosis. Taken together, our data imply a mandatory role for PtdIns 4-kinase and phosphoinositide products in the regulated exocytosis of SSV in mammalian nerve terminals.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/07/20 alle ore 19:36:34