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Titolo:
CLASS-SPECIFIC INHIBITION OF HOUSE-DUST MITE PROTEINASES WHICH CLEAVECELL-ADHESION, INDUCE CELL-DEATH AND WHICH INCREASE THE PERMEABILITY OF LUNG EPITHELIUM
Autore:
WINTON HL; WAN H; CANNELL MB; THOMPSON PJ; GARROD DR; STEWART GA; ROBINSON C;
Indirizzi:
ST GEORGE HOSP,SCH MED,DEPT PHARMACOL & CLIN PHARMACOL,CRANMER TERRACE LONDON SW17 0RE ENGLAND ST GEORGE HOSP,SCH MED,DEPT PHARMACOL & CLIN PHARMACOL LONDON SW17 0RE ENGLAND UNIV WESTERN AUSTRALIA,QUEEN ELIZABETH II MED CTR,DEPT MED NEDLANDS WA 6907 AUSTRALIA UNIV WESTERN AUSTRALIA,QUEEN ELIZABETH II MED CTR,DEPT MICROBIOL NEDLANDS WA 6907 AUSTRALIA UNIV MANCHESTER,SCH BIOL SCI MANCHESTER M13 9PT LANCS ENGLAND
Titolo Testata:
British Journal of Pharmacology
fascicolo: 6, volume: 124, anno: 1998,
pagine: 1048 - 1059
SICI:
0007-1188(1998)124:6<1048:CIOHMP>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLOW CYTOMETRIC DETECTION; DER-P-I; TIGHT JUNCTIONS; ANNEXIN-V; DERMATOPHAGOIDES-PTERONYSSINUS; PHOSPHATIDYLSERINE EXPRESSION; BRONCHIAL-MUCOSA; FAS LIGAND; APOPTOSIS; ALLERGEN;
Keywords:
BRONCHIAL EPITHELIUM; ASTHMA; ALLERGENS; APOPTOSIS; PROTEINASE INHIBITORS; TIGHT JUNCTIONS; DERMATOPHAGOIDES PTERONYSSINUS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
76
Recensione:
Indirizzi per estratti:
Citazione:
H.L. Winton et al., "CLASS-SPECIFIC INHIBITION OF HOUSE-DUST MITE PROTEINASES WHICH CLEAVECELL-ADHESION, INDUCE CELL-DEATH AND WHICH INCREASE THE PERMEABILITY OF LUNG EPITHELIUM", British Journal of Pharmacology, 124(6), 1998, pp. 1048-1059

Abstract

1 House dust mite (HDM) allergens with cysteine and serine proteinaseactivity are risk factors for allergic sensitization and asthma. A simple method to fractionate proteinase activity from HDM faecal pelletsinto cysteine and serine class activity is described. 2 Both proteinase fractions increased the permeability of epithelial cell monolayers. The effects of the serine proteinase fraction were inhibited by 4-(2-aminoethyl)-benzenesulphonyl fluoride hydrochloride (AEBSF) and soybean trypsin inhibitor (SBTI). The effects of the cysteine proteinase fraction could be inhibited by E-64. No reciprocity of action was found. 3 Treatment of epithelial monolayers with either proteinase fraction caused breakdown of tight junctions (TJs). AEBSF inhibited TJ breakdowncaused by the serine proteinase fraction, whereas E-64 inhibited the cysteine proteinase fraction. 4 Agarose gel electrophoresis revealed that the proteinases induced DNA cleavage which was inhibited by the matrix metalloproteinase inhibitor BB-250. Compound E-64 inhibited DNA fragmentation caused by the cysteine proteinase fraction, but was without effect on the serine proteinase fraction. Staining of proteinase-treated cells with annexin V (AV) and propidium iodide (PI) revealed a diversity of cellular responses. Some cells stained only with AV indicating early apoptosis, whilst others were dead and stained with both AVand PI. 5 HDM proteinases exert profound effects on epithelial cells which will promote allergic sensitization; namely disruption of intercellular adhesion, increased paracellular permeability and initiation of cell death. Attenuation of these actions by proteinase inhibitors leads to the conclusion that compounds designed to be selective for the HDM enzymes may represent a novel therapy for asthma.

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Documento generato il 25/11/20 alle ore 04:31:59