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Titolo:
BIOCHEMICAL-CHARACTERIZATION OF TELLURITE-REDUCING ACTIVITIES OF BACILLUS-STEAROTHERMOPHILUS-V
Autore:
MOSCOSO H; SAAVEDRA C; LOYOLA C; PICHUANTES S; VASQUEZ C;
Indirizzi:
UNIV SANTIAGO CHILE,FAC QUIM & BIOL,MOL MICROBIOL LAB SANTIAGO CHILE UNIV SANTIAGO CHILE,FAC QUIM & BIOL,MOL MICROBIOL LAB SANTIAGO CHILE CHIRON CORP,DEPT IMMUNODIAGNOST DEV EMERYVILLE CA 94608
Titolo Testata:
Research in microbiology (Paris)
fascicolo: 6, volume: 149, anno: 1998,
pagine: 389 - 397
SICI:
0923-2508(1998)149:6<389:BOTAOB>2.0.ZU;2-V
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESTRICTION-MODIFICATION SYSTEM; PLASMID-DETERMINED RESISTANCE; HEAVY-METAL RESISTANCE; ESCHERICHIA-COLI; MICROBIAL TRANSFORMATIONS; THERMOPHILIC BACTERIA; NUCLEOTIDE-SEQUENCE; PHYSIOLOGY; REDUCTION; PHH1508A;
Keywords:
TELLURITE; OXYANION; THERMOPHILIC BACTERIUM; REDUCTION; TOXICITY; RESISTANCE; H1 AND H2 FRACTIONS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
46
Recensione:
Indirizzi per estratti:
Citazione:
H. Moscoso et al., "BIOCHEMICAL-CHARACTERIZATION OF TELLURITE-REDUCING ACTIVITIES OF BACILLUS-STEAROTHERMOPHILUS-V", Research in microbiology (Paris), 149(6), 1998, pp. 389-397

Abstract

Bacillus stearothermophilus V is a naturally occurring Gram-positive rod which exhibits resistance to potassium tellurite, Crude extracts of this bacterium catalyse the NADH-dependent, protease-sensitive reduction of K2TeO3 in vitro. Two fractions which showed the ability to reduce potassium tellurite (H1 and H2) were obtained. Fraction H1 behavedas a macroaggregate exhibiting a very high molecular mass that could not be estimated accurately. Upon electrophoresis in polyacrylamide gels in the presence of SDS, however, it was resolved into three distinct bands of 60, 41 and 37.5 kDa. On the other hand, an M-r of 121 was determined for fraction H2 by means of gel filtration and high-pressureliquid chromatography. In SDS-PAGE a unique protein band of 60 kDa was observed, suggesting that it is actually a dimer. Both fractions showed pH and temperature optima of 7.5 and 57 degrees C, respectively. Concentrations of 2.5 M NaCl or 0.35 mM SDS inhibited fraction H2 almost completely, while fraction H1 retained 20% of its activity under thesame conditions. Concentrations of 5 mM EDTA caused the activity of both fractions to increase 2-fold. In addition to reducing tellurite, they were also able to reduce Na2SeO3 and Na2SO3 in vitro.

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Documento generato il 12/07/20 alle ore 03:13:50