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Titolo:
HUMAN PLASMA FIBRONECTIN MEDIATES ADHESION OF U937 CELLS BY RGD AND CS1
Autore:
KATOH K; MOHRI H; OGAWA K; OKUBO T;
Indirizzi:
YOKOHAMA CITY UNIV,SCH MED,DEPT INTERNAL MED 1,KANAZAWA KU,3-9 FUKUURA YOKOHAMA KANAGAWA 236 JAPAN YOKOHAMA CITY UNIV,SCH MED,DEPT INTERNAL MED 1,KANAZAWA KU YOKOHAMA KANAGAWA 236 JAPAN
Titolo Testata:
Journal of thrombosis and thrombolysis
fascicolo: 2, volume: 5, anno: 1998,
pagine: 129 - 134
SICI:
0929-5305(1998)5:2<129:HPFMAO>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
ARG-GLY-ASP; INHIBITS PLATELET-AGGREGATION; EXTRACELLULAR-MATRIX; INTEGRIN ALPHA-4-BETA-1; RECOGNITION SIGNAL; BINDING; IDENTIFICATION; RECEPTOR; SEQUENCE; ATTACHMENT;
Keywords:
FIBRONECTIN; RGDS; CS1; U937 CELLS; INTEGRIN RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
42
Recensione:
Indirizzi per estratti:
Citazione:
K. Katoh et al., "HUMAN PLASMA FIBRONECTIN MEDIATES ADHESION OF U937 CELLS BY RGD AND CS1", Journal of thrombosis and thrombolysis, 5(2), 1998, pp. 129-134

Abstract

Fibronectin specifically binds to U937 cells (monocytic cell line) ina dose-dependent manner. The specific receptors for the RGD sequence have been identified as alpha(5)beta(1) and alpha(IIb)beta(2), and that for CS1 has been defined as alpha(4)beta(1). RGDS, CS1 peptide, and two peptides together showed similar inhibitory activities on this adhesion, while the 29-kD dispase-digested fragment of the C-terminal heparin-binding domain did not. Thus, the adhesion of fibronectin to U937cells is mainly mediated by RGDS in the cell-binding domain and CS1 in the alternatively spliced region. Flow cytometry using monoclonal antibodies revealed expressions of alpha(3)beta(1), alpha(4)beta(1), andalpha(5)beta(1), and not expression of alpha(2)beta(1). Adhesion of U937 cells to fibronectin-coated wells is specific and is inhibited by anti-alpha(4)beta(1), and anti-alpha(5)beta(1) monoclonal antibodies. The IC50 for anti-alpha(5)beta(1) antibody was almost a log lower thanthe value for anti-alpha(4)beta(1) antibody. These results demonstrated that interactions of RGDS and CS1 sequence of fibronectin with alpha(5)beta(1) and alpha(4)beta(1) on U937 cells mere required for the adhesion of U937 cells to fibronectin. These results may provide furtherinformation to understand the mechanism(s) of tumor cell adhesion andatherogenesis.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 20:01:16