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Titolo:
RHOE REGULATES ACTIN CYTOSKELETON ORGANIZATION AND CELL-MIGRATION
Autore:
GUASCH RM; SCAMBLER P; JONES GE; RIDLEY AJ;
Indirizzi:
LUDWIG INST CANC RES,91 RIDING HOUSE ST LONDON W1P 8BT ENGLAND LUDWIG INST CANC RES LONDON W1P 8BT ENGLAND UNIV LONDON KINGS COLL,RANDALL INST,MUSCLE & MOTIL RES CTR LONDON WC2B 5RL ENGLAND INST CHILD HLTH LONDON WC1N 1EH ENGLAND UNIV LONDON UNIV COLL,DEPT BIOCHEM & MOL BIOL LONDON WC1E 6BT ENGLAND
Titolo Testata:
Molecular and cellular biology
fascicolo: 8, volume: 18, anno: 1998,
pagine: 4761 - 4771
SICI:
0270-7306(1998)18:8<4761:RRACOA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
BINDING PROTEIN-RHO; SCATTER FACTOR; FOCAL ADHESIONS; STRESS FIBERS; NEURITE RETRACTION; EFFECTOR REGION; RAS; KINASE; GTPASE; PHOSPHORYLATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
55
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Guasch et al., "RHOE REGULATES ACTIN CYTOSKELETON ORGANIZATION AND CELL-MIGRATION", Molecular and cellular biology, 18(8), 1998, pp. 4761-4771

Abstract

The actin cytoskeleton is regulated by Rho family proteins: in fibroblasts, Rho mediates the formation of actin stress fibers, whereas Rac regulates lamellipodium formation and Cdc42 controls filopodium formation. We have cloned the mouse RhoE gene, whose product is a member of the Rho family that shares (except in one amino acid) the conserved effector domain of RhoA, RhoB, and RhoC. RhoE is able to bind GTP but does not detectably bind GDP and has low intrinsic GTPase activity compared with Rac, The role of RhoE in regulating actin organization was investigated by microinjection in Bac1.2F5 macrophages and MDCK cells. In macrophages, RhoE induced actin reorganization, leading to the formation of extensions resembling filopodia and pseudopodia. In MDCK cells, RhoE induced the complete disappearance of stress fibers, together with cell spreading. However, RhoE did not detectably affect the actin bundles that run parallel to the outer membranes of cells at the periphery of colonies, which are known to be dependent on RhoA. In addition, RhoE induced an increase in the speed of migration of hepatocyte growth factor/scatter factor-stimulated MDCK cells, in contrast to the previously reported inhibition produced by activated RhoA. The subcellular localization of RhoE at the lateral membranes of MDCK cells suggests a role in cell-cell adhesion, as has been shown for RhoA, These results suggest that RhoE may act to inhibit signalling downstream of RhoA, altering some RhoA-regulated responses, such as stress fiber formation, but not affecting others, such as peripheral actin bundle formation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/07/20 alle ore 09:27:32