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Titolo:
3'-PROCESSED MESSENGER-RNA IS PREFERENTIALLY TRANSLATED IN CHLAMYDOMONAS-REINHARDTII CHLOROPLASTST
Autore:
ROTT R; LEVY H; DRAGER RG; STERN DB; SCHUSTER G;
Indirizzi:
TECHNION ISRAEL INST TECHNOL,DEPT BIOL IL-32000 HAIFA ISRAEL TECHNION ISRAEL INST TECHNOL,DEPT BIOL IL-32000 HAIFA ISRAEL CORNELL UNIV,BOYCE THOMPSON INST PLANT RES ITHACA NY 14853
Titolo Testata:
Molecular and cellular biology
fascicolo: 8, volume: 18, anno: 1998,
pagine: 4605 - 4611
SICI:
0270-7306(1998)18:8<4605:3MIPTI>2.0.ZU;2-C
Fonte:
ISI
Lingua:
ENG
Soggetto:
3' INVERTED REPEATS; MESSENGER-RNA; GENE-EXPRESSION; TRANSCRIPTION; PROTEINS; BINDING; SPINACH; COMPLEX; INVIVO; MATURATION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
R. Rott et al., "3'-PROCESSED MESSENGER-RNA IS PREFERENTIALLY TRANSLATED IN CHLAMYDOMONAS-REINHARDTII CHLOROPLASTST", Molecular and cellular biology, 18(8), 1998, pp. 4605-4611

Abstract

3'-end processing of nucleus-encoded mRNAs includes the addition of apoly(A) tail that is important for translation initiation. Since the vast majority of chloroplast mRNAs acquire their 3' termini by processing yet are not polyadenylated, we asked whether 3' end maturation plays a role in chloroplast translation. A general characteristic of the 3' untranslated regions of chloroplast mRNAs is an inverted repeat (LR) sequence that can fold into a stem-loop structure. These stem-loops and their flanking sequences serve as RNA 3'-end formation signals. Deletion of the Chlamydomonas chloroplast atpB 3' IR in strain Delta 26 results in reduced accumulation of atpB transcripts and the chloroplast ATPase beta-subunit, leading to weakly photosynthetic growth. Of theresidual atpB mRNA in Delta 26, approximately 1% accumulates as a discrete RNA of wild-type size, while the remainder is heterogeneous in length due to the lack of normal 3' end maturation. In this work, we have analyzed whether these unprocessed atpB transcripts are actively translated in vivo. We found that only the minority population of discrete transcripts of wild-type size is associated with polysomes and thusaccounts for the ATPase beta-subunit which accumulates in Delta 26. Analysis of chloroplast rbcL mRNA revealed that transcripts extending beyond the mature 3' end were not polysome associated. These results suggest that 3'-end processing of chloroplast mRNA is required for or strongly stimulates its translation.

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Documento generato il 25/01/20 alle ore 18:36:22