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Titolo:
DETERMINATION OF TOTAL CYSTEAMINE IN HUMAN SERUM BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION
Autore:
STACHOWICZ M; LEHMANN B; TIBI A; PROGNON P; DAURAT V; PRADEAU D;
Indirizzi:
AP HP,PHARM CENT HOP,SECTEUR ESSAIS CLIN,7 RUE FER MOULIN F-75005 PARIS FRANCE AP HP,PHARM CENT HOP,SECTEUR ESSAIS CLIN F-75005 PARIS FRANCE AP HP,PHARM CENT HOP,LAB CENT ANAL F-75005 PARIS FRANCE AP HP,HOP ST LOUIS,DELEGAT RECH CLIN F-75010 PARIS FRANCE
Titolo Testata:
Journal of pharmaceutical and biomedical analysis
fascicolo: 4-5, volume: 17, anno: 1998,
pagine: 767 - 773
SICI:
0731-7085(1998)17:4-5<767:DOTCIH>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLAME PHOTOMETRIC DETECTION; FLUOROMETRIC DETECTION; GAS-CHROMATOGRAPHY; GLUTATHIONE; CYSTINOSIS; MONOBROMOBIMANE; THIOLS; ASSAY;
Keywords:
CLINICAL TRIAL; CYSTEAMINE; PHOSPHOCYSTEAMINE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; FLUOROMETRIC DETECTION; MONOBROMOBIMANE; BIOAVAILABILITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
18
Recensione:
Indirizzi per estratti:
Citazione:
M. Stachowicz et al., "DETERMINATION OF TOTAL CYSTEAMINE IN HUMAN SERUM BY A HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH FLUORESCENCE DETECTION", Journal of pharmaceutical and biomedical analysis, 17(4-5), 1998, pp. 767-773

Abstract

A convenient, reliable and rapid method for the determination of total cysteamine in human plasma by high-performance liquid chromatographywith fluorescence detection is reported. This assay involves reduction of samples with dithiothreitol, derivatization of total cysteamine by addition of monobromobimane and protein precipitation by perchloric acid. The calibration curve was linear in the range 2-150 nmol ml(-1) and the detection limit was 0.5 nmol ml(-1). This method was successfully applied for a pharmacokinetic study of three cysteamine derivatives in healthy volunteers without any interference from coexisting substances. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 31/03/20 alle ore 04:35:01