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Titolo:
HIGH-LEVELS OF EXPRESSION OF COLLAGENASE-3 (MMP-13) IN PATHOLOGICAL CONDITIONS ASSOCIATED WITH A FOREIGN-BODY REACTION
Autore:
IMAI S; KONTTINEN YT; JUMPPANEN M; LINDY O; CEPONIS A; KEMPPINEN P; SORSA T; SANTAVIRTA S; XU JW; LOPEZOTIN C;
Indirizzi:
UNIV HELSINKI,INST BIOMED,DEPT ANAT & MED CHEM,POB 9,SILTAVUORENPENGER 20A FIN-0014 HELSINKI FINLAND UNIV HELSINKI,INST BIOMED,DEPT ANAT & MED CHEM FIN-0014 HELSINKI FINLAND INVALID FDN,ORTON RES INST FIN-00280 HELSINKI FINLAND UNIV HELSINKI,CENT HOSP,DEPT ORTHOPAED & TRAUMATOL FIN-00260 HELSINKIFINLAND UNIV OVIEDO,DEPT BIOCHEM & MOL BIOL E-33006 OVIEDO SPAIN
Titolo Testata:
Journal of bone and joint surgery. British volume
fascicolo: 4, volume: 80B, anno: 1998,
pagine: 701 - 710
SICI:
0301-620X(1998)80B:4<701:HOEOC(>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
HUMAN OSTEOARTHRITIC CARTILAGE; TOTAL HIP REPLACEMENTS; MATRIX METALLOPROTEINASES; SYNOVIAL-MEMBRANE; DESTRUCTIVE ARTHROPATHY; COLLAGENOLYTIC ACTIVITY; PROLYL 4-HYDROXYLASE; OSTEO-ARTHRITIS; CLONING; CHONDROCALCINOSIS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
36
Recensione:
Indirizzi per estratti:
Citazione:
S. Imai et al., "HIGH-LEVELS OF EXPRESSION OF COLLAGENASE-3 (MMP-13) IN PATHOLOGICAL CONDITIONS ASSOCIATED WITH A FOREIGN-BODY REACTION", Journal of bone and joint surgery. British volume, 80B(4), 1998, pp. 701-710

Abstract

A foreign-body-type host response can contribute to the induction andrelease of collagenolytic tissue-destructive enzymes of pathogenetic significance. Our aim was to analyse collagenase-3 in two conditions with putative involvement of foreign-body reactions. Synovial membrane-like tissue samples mere obtained from cases of aseptic loosening of atotal hip replacement (THR) and osteoarthritis (OA). The reverse transcription polymerase chain reaction (RT-PCR) disclosed that all the samples from patients contained collagenase-3 mRNA compared with only three out of ten control samples. The identity of the RT-PCR amplification product was confirmed by nucleotide sequencing. Immunohistochemicalstaining showed that collagenase-3 was present in endothelial cells, macrophages and fibroblasts, including those found in the synovial lining. This finding was confirmed by avidin-biotin-peroxidase complex-alkaline phosphatase-anti-alkaline phosphatase double staining and the specificity of the staining by antigen preabsorption using recombinant human collagenase-3. Collagenase-3 was released into the extracellularspace and thus found in the synovial fluid in all patient samples as shown by Western blotting, The similar extent of collagenase-3 expression in aseptic loosening and OA compared with the low expression in control synovial membrane suggests involvement of a similar, foreign-body-based pathogenetic component in both, Comparative analysis of collagenase-3 and of foreign particles indicates that paracrine factors rather than phagocytosis per se are responsible for the induction of collagenase3. We suggest that due to its localisation and substrate specificity, collagenase-3 may play a significant pathogenetic role in accelerating tissue destruction in OA and in aseptic loosening of a THR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 21/09/20 alle ore 09:30:10