Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
THE TUMOR NECROSIS FACTOR-SENSITIVE POOL OF SPHINGOMYELIN IS RESYNTHESIZED IN A DISTINCT COMPARTMENT OF THE PLASMA-MEMBRANE
Autore:
ANDRIEUABADIE N; CARPENTIER S; SALVAYRE R; LEVADE T;
Indirizzi:
CHU RANGUEIL,INST LOUIS BUGNARD,LAB BIOCHIM MALAD METAB,INSERM,U466,BAT L3,1 AVE JEAN POULHES F-31403 TOULOUSE 4 FRANCE CHU RANGUEIL,INST LOUIS BUGNARD,LAB BIOCHIM MALAD METAB,INSERM,U466 F-31403 TOULOUSE 4 FRANCE
Titolo Testata:
Biochemical journal
, volume: 333, anno: 1998,
parte:, 1
pagine: 91 - 97
SICI:
0264-6021(1998)333:<91:TTNFPO>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANINE KIDNEY CELLS; FACTOR-KAPPA-B; SPHINGOLIPID METABOLISM; CULTURED FIBROBLASTS; SIGNALING PATHWAY; GOLGI-APPARATUS; CERAMIDE; ACTIVATION; CYCLE; PHOSPHATIDYLCHOLINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
50
Recensione:
Indirizzi per estratti:
Citazione:
N. Andrieuabadie et al., "THE TUMOR NECROSIS FACTOR-SENSITIVE POOL OF SPHINGOMYELIN IS RESYNTHESIZED IN A DISTINCT COMPARTMENT OF THE PLASMA-MEMBRANE", Biochemical journal, 333, 1998, pp. 91-97

Abstract

Sphingomyelin (SM) biosynthesis is believed to occur in the early Golgi apparatus, plasma membrane and recycling endosomes. In the present study, the localization of the SM synthesis that follows its hydrolysis upon activation of the SM signal-transduction pathway was investigated in human skin fibroblasts treated with tumour necrosis factor (TNF)alpha. After TNF alpha-induced degradation, the intracellular SM levels returned to baseline levels within 30-60 min in cells treated at 37degrees C. Pretreatment or co-incubation of cells with bacterial sphingomyelinase or phospholipase C, decreasing the SM and phosphatidylcholine content in the external leaflet of the plasma membrane respectively, did not inhibit SM resynthesis. However, SM resynthesis was not observed when TNF alpha-treated cells were continuously exposed to exogenous sphingomyelinase, suggesting that under these particular conditions the resynthesized SM becomes accessible to the enzyme. Furthermore,whereas inhibition of vesicular traffic/endocytosis at 4 degrees C blocked exoplasmic SM resynthesis, it did not alter SM resynthesis in TNF alpha-treated fibroblasts, negating the role of endosomes and the Golgi apparatus. This was further evidenced by the finding that after SMresynthesis, TNF alpha was again able to promote SM turnover, even at4 degrees C. In addition, when the exoplasmic leaflet SM was hydrolysed by treating fibroblasts with bacterial sphingomyelinase, resynthesis of SM occurred at 37 degrees C much more slowly than after TNF alphatreatment. These findings support strongly the conclusion that the SM, which is resynthesized after TNF alpha-induced hydrolysis, resides in the cytosolic leaflet of the plasma membrane, and that the process involved in this resynthesis displays characteristics different from those of the previously described SM synthases.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/06/20 alle ore 01:15:32