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Titolo:
DIFFERENTIAL-EFFECTS OF PHORBOL ESTER ON GROWTH AND PROTEIN-KINASE-C ISOENZYME REGULATION IN HUMAN HEPATOMA HEP3B CELL
Autore:
HSU SL; CHOU YH; YIN SC; LIU JY;
Indirizzi:
TAICHUNG VET GEN HOSP,DEPT EDUC & RES,SECT 3,160,CHUNG GANG RD TAICHUNG 40705 TAIWAN CHUNG SHAN MED & DENT COLL,INST BIOCHEM TAICHUNG 402 TAIWAN
Titolo Testata:
Biochemical journal
, volume: 333, anno: 1998,
parte:, 1
pagine: 57 - 64
SICI:
0264-6021(1998)333:<57:DOPEOG>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
SIGNAL-TRANSDUCTION; CARCINOMA CELLS; RAT FIBROBLASTS; DOWN-REGULATION; BETA-II; EXPRESSION; ALPHA; TRANSLOCATION; ACTIVATION; ISOFORMS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
S.L. Hsu et al., "DIFFERENTIAL-EFFECTS OF PHORBOL ESTER ON GROWTH AND PROTEIN-KINASE-C ISOENZYME REGULATION IN HUMAN HEPATOMA HEP3B CELL", Biochemical journal, 333, 1998, pp. 57-64

Abstract

PMA has both mitogenic and antiproliferative effects on human hepatoma Hep3B cells. In response to low PMA concentration (10 nM), Hep3B cells displayed an increasing proliferation potentiation. At high PMA concentration (1 mu M) Hep3B cells exhibited modest cytostatic effects. Determinations of protein kinase C (PK(C) activity in PMA-treated cellsrevealed that alterations in PKC activity are associated with proliferative capacity. The decrease in PKC activity mediated by a high dose of PMA was accompanied by cell growth inhibition. Increases in PKC activity mediated by a low dose of PMA were consistent with proliferationstimulation. Immunoblot analysis showed that there are at least six PKC isoenzymes: alpha, delta, epsilon, mu, zeta and iota/lambda, constitutively expressed in Hep3B cells. Cellular fractionation and immunocytochemical staining results demonstrated that both 10 nM and 1 mu M PMA treatments induced a marked translocation of PKC-alpha from cytosol to membrane or nuclear fraction within 5-30 min. At the same time PKC-delta and epsilon were translocated from the membrane to nuclear fraction. In addition, prolonged treatment with 1 mu M PMA, but not with 10nM PMA, selectively mediated the down-regulation of these three PKC isoenzymes. The distinct effects of different concentrations of PMA on cell proliferation and PKC-alpha, delta and epsilon isoenzyme modulation support the involvement of these three PKC isotypes in the mechanism of action of Hep3B cells in cell growth events.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/20 alle ore 22:08:55