Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
IMPAIRED MECHANICAL STABILITY, MIGRATION AND CONTRACTILE CAPACITY IN VIMENTIN-DEFICIENT FIBROBLASTS
Autore:
ECKES B; DOGIC D; COLUCCIGUYON E; WANG N; MANIOTIS A; INGBER D; MERCKLING A; LANGA F; AUMAILLEY M; DELOUVEE A; KOTELIANSKY V; BABINET C; KRIEG T;
Indirizzi:
UNIV COLOGNE,DEPT DERMATOL D-50924 COLOGNE GERMANY INST BIOL & CHIM PROT,CNRS UPR 412 F-69367 LYON 07 FRANCE INST PASTEUR,CNRS URA 1960,UNITE BIOL DEV F-75724 PARIS 15 FRANCE HARVARD UNIV,SCH PUBL HLTH BOSTON MA 02115 CHILDRENS HOSP BOSTON MA 02115 HARVARD UNIV,SCH MED BOSTON MA 02115 INST CURIE,CNRS UMR 144 F-75231 PARIS 05 FRANCE
Titolo Testata:
Journal of Cell Science
, volume: 111, anno: 1998,
parte:, 13
pagine: 1897 - 1907
SICI:
0021-9533(1998)111:<1897:IMSMAC>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
3-DIMENSIONAL COLLAGEN LATTICES; INTERMEDIATE FILAMENTS; TRANSGENIC MICE; MOUSE EMBRYO; CELL-SHAPE; EXTRACELLULAR-MATRIX; EXPRESSION; CYTOSKELETON; GENE; MICROTUBULES;
Keywords:
CYTOSKELETON; COLLAGEN LATTICE; TENSEGRITY; MECHANOTRANSDUCTION; IN VITRO WOUND HEALING;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
52
Recensione:
Indirizzi per estratti:
Citazione:
B. Eckes et al., "IMPAIRED MECHANICAL STABILITY, MIGRATION AND CONTRACTILE CAPACITY IN VIMENTIN-DEFICIENT FIBROBLASTS", Journal of Cell Science, 111, 1998, pp. 1897-1907

Abstract

Loss of a vimentin network due to gene disruption created viable micethat did not differ overtly from wild-type littermates. Here,. primary fibroblasts derived from vimentin-deficient (-/-) and wild-type (+/+) mouse embryos were cultured, and biological functions were studied in in vitro systems resembling stress situations. Stiffness of -/- fibroblasts was reduced by 40% in comparison to wild-type cells. Vimentin-deficient cells also displayed reduced mechanical stability, motility and directional migration towards different chemo-attractive stimuli. Reorganization of collagen fibrils and contraction of collagen lattices were severely impaired. The spatial organization of focal contact proteins, as well as actin microfilament organization was disturbed. Thus, absence of a vimentin filament network does not impair basic cellular functions needed for growth in culture, but cells are mechanically less stable, and we propose that therefore they are impaired in all functions depending upon mechanical stability.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 15:45:58