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Titolo:
BACTERIAL LIPOPOLYSACCHARIDE CONTAMINATION OF COMMERCIAL COLLAGEN PREPARATIONS MAY MEDIATE DENDRITIC CELL MATURATION IN CULTURE
Autore:
SURI RM; AUSTYN JM;
Indirizzi:
UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT SURG OXFORD OX3 9DU ENGLAND UNIV OXFORD,JOHN RADCLIFFE HOSP,NUFFIELD DEPT SURG OXFORD OX3 9DU ENGLAND
Titolo Testata:
Journal of immunological methods
fascicolo: 1-2, volume: 214, anno: 1998,
pagine: 149 - 163
SICI:
0022-1759(1998)214:1-2<149:BLCOCC>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
EPIDERMAL LANGERHANS CELLS; COLONY-STIMULATING FACTOR; TUMOR-NECROSIS-FACTOR; ADHESION MOLECULES; HUMAN BLOOD; MONOCLONAL-ANTIBODIES; NONLYMPHOID TISSUES; MOUSE SPLEEN; IFN-GAMMA; TNF-ALPHA;
Keywords:
DENDRITIC CELL; MATURATION; COLLAGEN; LIPOPOLYSACCHARIDE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Science Citation Index Expanded
Citazioni:
75
Recensione:
Indirizzi per estratti:
Citazione:
R.M. Suri e J.M. Austyn, "BACTERIAL LIPOPOLYSACCHARIDE CONTAMINATION OF COMMERCIAL COLLAGEN PREPARATIONS MAY MEDIATE DENDRITIC CELL MATURATION IN CULTURE", Journal of immunological methods, 214(1-2), 1998, pp. 149-163

Abstract

Dendritic cells (DC) are potent antigen presenting cells, which are responsible for the initiation of naive T and T-dependent immune responses. The present studies were based upon recent reports that commercial collagen I preparations induce the maturation of human DC in vitro. We show that human blood monocyte-derived (GM-CSF and IL-4 cultured) DC pulsed on collagen I-coated plates undergo a dose-dependent increasein stimulatory capacity in oxidative mitogenesis assays. This is accompanied by the upregulation of costimulatory molecules (CD40, CD80, CD86), CD25, ICAM-1 and the DC-specific marker CD83. The maturation effect is more potent than TNF-alpha, which is a known mediator of DC function. However, bacterial lipopolysaccharide (LPS), a powerful inducer of DC maturation, was found to be present at very high levels in one commercial collagen solution that was tested. The effect of LPS upon DCmaturation was similar to culture with collagen. Furthermore, a different collagen I preparation with low levels of LPS contamination was less effective at inducing DC maturation, while spiking the collagen solution with LPS prior to plastic coating equalised these effects. Finally, human monocyte-derived DC were found not to express typical collagen receptors VLA-1, 2 and 3. We therefore propose that LPS contamination may at least partially explain reported collagen I induced DC maturation. (C) 1998 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 18/09/20 alle ore 17:17:39